BCAM/CD239 Antibody (87202)

• Reactivity: Hu
• Applications: WB
• Clone: 87202
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

BCAM/CD239 Antibody (87202) Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human BCAM
  Glu32-Ala547
  Accession # P50895
• Specificity: Detects human BCAM in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human (rh) ALCAM, rhEpCAM, recombinant mouse (rm) MAdCAM, rhMCAM, rhNCAM-L1, rmOCAM, or rmTROP-2 is observed.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: BCAM
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for BCAM/CD239 Antibody (87202)

• antigen identified by monoclonal F8
• Auberger B antigen
• basal cell adhesion molecule (Lu and Au blood groups)
• basal cell adhesion molecule (Lutheran blood group)
• basal cell adhesion molecule
• B-CAM cell surface glycoprotein
• BCAM
• B-cell adhesion molecule
• CD239 antigen
• CD239
• F8/G253 antigen
• glycoprotein 95kDa
• LUAU
• Lutheran antigen
• Lutheran blood group (Auberger b antigen included)
• Lutheran blood group glycoprotein
• MSK19

Background

Basal-Cell Adhesion Molecule (BCAM) and Lutheran blood group glycoprotein (LU) are two alternatively spliced variants of a single immunoglobulin superfamily (IgSF) protein that differ in the length of their cytoplasmic tails. BCAM cDNA encodes a 628 amino acid (aa) precursor protein with a putative 31 aa signal peptide, a 597 aa extracellular domain containing three C2 type and two V-type Ig like domains, a 21 aa transmembrane domain, and a 19 aa cytoplasmic domain. Compared to the 40 aa cytoplasmic domain present in LU, the BCAM cytoplasmic tail lacks the putative Src homology 3 (SH3) binding site that may be involved in mediating intracellular signaling. BCAM/LU has wide tissue distribution and is expressed on erythrocytes, the endothelium of blood vessels and on the basal layer of cells in the epithelia. The expression of BCAM/LU in normal tissues is higher in fetal versus adult tissues. BCAM/LU expression is also upregulated in sickle cell disease red blood cells, in activated keratinocytes and following malignant transformation in some cell types in vivo and in vitro. BCAM/LU has been shown to be an adhesion molecule that binds laminin, a basement membrane protein involved in cell differentiation, adhesion, migration and proliferation.

1. Campbell, I.G. et al. (1994) Cancer Research 54:5761.
2. Parsons, S.F. et al. (1995) Proc. Natl. Acad. Sci. USA 92:5496.
3. Udani, M. et al. (1998) J. Clin. Invest. 101:2550.
4. Schon, M. et al. (2000) J. Invest. Dermatol. 115:1047.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for BCAM/CD239 Antibody (MAB148)(2)

Publications using MAB148

• De Grandis , Maria, Cambot , Marie, Wautier , Marie-Pa, Cassinat , Bruno, Chomienne , Christin, Colin , Yves, Wautier , Jean-Luc, Le Van Kim , Caroline, El Nemer , Wassim JAK2V617F activates Lu/BCAM-mediated red cell adhesion in polycythemia vera through an EpoR-independent Rap1/Akt pathway. Blood, 2012-11-16;121(4):658-65. 2012-11-16 [PMID: 23160466] (Flow Cytometry, Human)
• Kikkawa Y, Miwa T, Tohara Y, Hamakubo T, Nomizu M An antibody to the lutheran glycoprotein (Lu) recognizing the LU4 blood type variant inhibits cell adhesion to laminin alpha5. PLoS ONE, 2011-08-12;6(8):e23329. 2011-08-12 [PMID: 21858073] (Epitope Mapping, Human)

Bioinformatics

• Gene Symbol: BCAM
• Uniprot:
  • P50895()