Bassoon Antibody (L124/59) - Chimeric - BSA Free

Representative section of formalin fixed, paraffin-embedded rat brain cerebellum region stained with NBP3-52352 at 1:100 dilution. DAPI nuclear stain (blue) shows cell nuclei. This antibody stains the presynaptic sites on Purkinje cell dendrites in the molecular layer of the cerebellum. Magnification 100X.

Representative section of formalin fixed, paraffin-embedded rat brain cerebellum region stained with NBP3-52352 at 1:100 dilution. DAPI nuclear stain (blue) shows cell nuclei. This antibody stains the presynaptic sites on Purkinje cell dendrites in the molecular layer of the cerebellum. Magnification 400X.

• Reactivity: Mu, Rt
• Applications: WB, ELISA, ICC/IF, IHC
• Clone: L124/59
• Clonality: Monoclonal
• Host: Chicken
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

Bassoon Antibody (L124/59) - Chimeric - BSA Free Summary

• Description: This recombinant antibody is a chimeric antibody created by replacing the mouse heavy and light constant regions of clone L124/59 with chicken IgY heavy and light constant regions. As such this antibody retains the same binding performance as the original clone L124/59 but can be detected using standard anti-chicken secondary antibodies allowing flexibility for multiplexing applications. This antibody is expressed recombinantly in HEK293 cells and then affinity purified from the cell culture media.
• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: Fusion protein amino acids 157-517 (zinc finger domains) of rat Bassoon (Uniprot#: O88778) produced recombinantly in E. Coli
• Isotype: IgY
• Clonality: Monoclonal
• Host: Chicken
• Gene: BSN
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Immunohistochemistry-Paraffin 1:100
  • Western Blot 1:500-1:1000
• Application Notes: 400 kDa for full-length protein, many smaller bands possible depending on sample used

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 1X PBS (pH 7.4)
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Affinity purified

Alternate Names for Bassoon Antibody (L124/59) - Chimeric - BSA Free

• bassoon (presynaptic cytomatrix protein)

Background

Bassoon is a 420 kDa protein that is a localized at the presynaptic nerve terminals and is believed to play a role in the structural and functional organization of the synaptic vesicle cycle (1-3). Bassoon does not belong to any known protein families. It has been found in rat and mouse with sequence identity of the two proteins at 96% (1). The human BASSOON gene has recently been cloned and localized (3). Bassoon is predicted to contain two double-zinc fingers, three coiled-coil regions, and two polyglutamine domains (1). The polyglutamine domains in the C-terminus are of interest, since it is known that for some human proteins, such as Huntingtin, abnormal amplification of this region can cause late-onset neurodegeneration (1,3). Bassoon is concentrated at sites opposite to postsynaptic densities in synaptic terminals and in cultured neurons, it is found to colocalize with GABA (A) and glutamate (GluR1) receptors (2). Another presynaptic protein, Piccolo (4) was found to colocalize with Bassoon in cultured hippocampal neurons (1). These observations suggested that they serve specific functions at synaptic junctions and may be involved in organization of the cytoskeleton at the site of neurotransmitter release (1).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

FAQs for Bassoon Antibody (NBP3-52352). (Showing 1 - 1 of 1 FAQ).

1. I am looking for a good antibody to detect Bassoon in mouse brain slices (PFA fixed); can you recommend any one for this?
   • After searching through our product catalog list, we have found the following antibodies which may be of interest: Bassoo. Using the filters on the left column of the site should allow you to filter by species and desired application. Any antibody that is listed to show validation for use in IHC-P and has also been validated in mouse samples will be backed by our 100% Guarantee. Under the terms and conditions of the guarantee, Novus will provide you with a full refund or replacement should the antibody fail to perform, as specified, in samples expressing the protein.

Bioinformatics

• Gene Symbol: BSN