BAIAP2 Antibody - BSA Free

Western Blot: BAIAP2 Antibody [NBP2-15545] - Various tissue extracts (50 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with BAIAP2 antibody diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.

Immunocytochemistry/Immunofluorescence: BAIAP2 Antibody [NBP2-15545] - Confocal immunofluorescence analysis of methanol-fixed A431, using BAIAP2 antibody (Green) at 1:500 dilution. Alpha-tubulin filaments are labeled with Alpha-tubulin antibody (Red) at 1:2000.

Immunohistochemistry-Paraffin: BAIAP2 Antibody [NBP2-15545] - Paraffin-embedded mouse brain. BAIAP2 antibody diluted at 1:500.

Western Blot: BAIAP2 Antibody [NBP2-15545] - Sample (30 ug of whole cell lysate) A: SK-N-SH 7.5% SDS PAGE diluted at 1:2000

• Reactivity: Hu, Mu, Rt, Bv
• Applications: WB, ICC/IF, IHC, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

BAIAP2 Antibody - BSA Free Summary

• Immunogen: Recombinant protein encompassing a sequence within the center region of human BAIAP2. The exact sequence is proprietary.
• Localization: Cytoplasm; Membrane; Cell projection; filopodium; ruffle
• Predicted Species: Bovine (98%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: BAIAP2
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Immunoprecipitation
  • Western Blot 1:500-1:20000
• Theoretical MW: 61 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: 0.1M Tris (pH 7), 0.1M Glycine, 20% Glycerol
• Preservative: 0.01% Thimerosal
• Purity: Antigen Affinity-purified

Alternate Names for BAIAP2 Antibody - BSA Free

• BAI1-associated protein 2IRS-58
• BAI-associated protein 2
• BAP2
• brain-specific angiogenesis inhibitor 1-associated protein 2
• Fas ligand-associated factor 3
• FLAF3
• Insulin receptor substrate p53
• Insulin receptor substrate p53/p58
• Insulin receptor substrate protein of 53 kDa
• IRSP53
• IRSp53/58
• Protein BAP2

Background

The protein encoded by this gene has been identified as a brain-specific angiogenesis inhibitor (BAI1)-binding protein. This adaptor protein links membrane bound G-proteins to cytoplasmic effector proteins. This protein functions as an insulin receptor tyrosine kinase substrate and suggests a role for insulin in the central nervous system. It also associates with a downstream effector of Rho small G proteins, which is associated with the formation of stress fibers and cytokinesis. This protein is involved in lamellipodia and filopodia formation in motile cells and may affect neuronal growth-cone guidance. This protein has also been identified as interacting with the dentatorubral-pallidoluysian atrophy gene, which is associated with an autosomal dominant neurodegenerative disease. Alternative splicing results in multiple transcript variants encoding distinct isoforms.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Bioinformatics

• Gene Symbol: BAIAP2
• Uniprot:
  • Q9UQB8(Human)