BAI3 Antibody (409611) [Unconjugated]

HEK293 human embryonic kidney cell line transfected with either (A) human BAI3 or (B) irrelevant transfectants and eGFP was stained with Mouse Anti-Human BAI3 Monoclonal Antibody (Catalog # MAB3965) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). Quadrant markers were set based on control antibody staining (Catalog # MAB002). View our protocol for Staining Membrane-associated Proteins.

• Reactivity: Hu
• Applications: WB, Flow, CyTOF-ready
• Clone: 409611
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

BAI3 Antibody (409611) [Unconjugated] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human BAI3
  Ala25-Thr880
  Accession # O60242
• Specificity: Detects human BAI3 in direct ELISAs and Western blots.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: BAI3
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 0.25 ug/10^6 cells
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for BAI3 Antibody (409611) [Unconjugated]

• BAI3
• brain-specific angiogenesis inhibitor 3
• dJ91B17.1 (brain-specific angiogenesis inhibitor 3)
• KIAA0550MGC133100

Background

Human BAI3 (brain-specific angiogenesis inhibitor 3) is a 177 kDa, 7-transmembrane (TM) member of the secretin receptor family. It is synthesized by neurons of the CNS and likely is a negative regulator of angiogenesis. BAI3 is 1498 amino acids (aa) in size. It contains three distinct regions; an N-terminal extracellular domain (ECD) (aa 25‑883), a 7-TM segment, and a C-terminal cytoplasmic region. The ECD contains four antiangiogenic TSP type1 repeats (aa 296‑508), and one GPS domain (aa 816‑867) that is likely used to cleave the ECD from the membrane-bound receptor. There is one alternate splice form that shows a deletion of aa 643‑665. Over aa 25‑880, human BAI3 shares 98% aa identity with mouse BAI3.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: BAI3
• Uniprot:
  • O60242()