BAFF/BLyS/TNFSF13B Antibody [Unconjugated]

BAFF/BLyS/TNFSF13B was detected in formalin fixed paraffin-embedded sections of human spleen using 15 µg/mL Goat Anti-Human BAFF/BLyS/TNFSF13B Antigen Affinity-purified Polyclonal Antibody (Catalog # AF124) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

In the presence of Goat F(ab')2 Anti-mouse IgM (1 µg/mL), Recombinant Human BAFF/BLyS/TNFSF13B (Catalog # 2149-BF) stimulates proliferation in mouse B cells in a dose-dependent manner (orange line). Under these conditions, proliferation elicited by Recombinant Human BAFF/BLyS/TNFSF13B (5 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human BAFF/BLyS/TNFSF13B Antigen Affinity-purified Polyclonal Antibody (Catalog # AF124). The ND50 is typically3‑12 ng/mL.

• Reactivity: Hu
• Applications: WB, IHC, B/N
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

BAFF/BLyS/TNFSF13B Antibody [Unconjugated] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human BAFF/BLyS/TNFSF13B
  Ala134-Leu285
  Accession # Q9Y275
• Specificity: Detects human BAFF/BLyS/TNFSF13B in direct ELISAs and Western blots. In direct ELISAs, less than 15% cross-reactivity with recombinant murine BAFF and less than 1% cross‑reactivity with recombinant human (rh) APRIL is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: TNFSF13B
• Endotoxin Note: <0.60 EU per 1 μg of the antibody by the LAL method.

Applications/Dilutions

• Dilutions:
  • Western Blot 0.1 ug/mL
  • Immunohistochemistry 5-15 ug/mL
  • Neutralization 3-12 ng/mL
• Control:

  BAFF/BLyS/TNFSF13B Knockout A549 Cell Lysate

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for BAFF/BLyS/TNFSF13B Antibody [Unconjugated]

• ApoL related ligand TALL-1
• B lymphocyte stimulator
• BAFF
• BAFFB-cell activating factor
• B-cell-activating factor
• BLyS
• BLYSB-lymphocyte stimulator
• CD257 antigen
• CD257
• Dendritic cell-derived TNF-like molecule
• DTL
• TALL1
• TALL-1delta BAFF
• TALL1Delta4 BAFF
• THANK
• TNF- and APOL-related leukocyte expressed ligand 1
• TNF and ApoL-related leukocyte expressed ligand 1
• TNF homolog that activates apoptosis
• TNFSF13B
• TNFSF20
• tumor necrosis factor (ligand) superfamily, member 13b
• tumor necrosis factor (ligand) superfamily, member 20
• tumor necrosis factor ligand superfamily member 13B
• tumor necrosis factor superfamily, member 13b
• tumor necrosis factor-like protein ZTNF4
• ZTNF4

Background

BAFF (also known as TALL-1, BLyS, and THANK) is a type II transmembrane glycoprotein belonging to the TNF superfamily and has been designated as TNF superfamily member 13B (TNFSF13B). Human BAFF is a 285 amino acid (aa) protein consisting of a 218 aa extracellular domain, a 21 aa transmembrane region and a 46 aa cytoplasmic tail (1, 2). BAFF has the typical structural characteristics of the TNF superfamily ligands. It is a homotrimeric protein having the structurally conserved motif known as TNF homology domain (3, 4). A higher ordered structure composed of a cluster of trimeric units resembling the structure of a viral capsid has also been reported (4). Human BAFF may be shed from the cell surface by proteolytic cleavage between R133 and Ala134 to yield a soluble form of the protein that is detectable in serum (1, 5). Within the TNF superfamily BAFF shares the highest homology (48%) with APRIL (1). BAFF shares with APRIL the ability to bind to BCMA and TACI and also binds specifically to BAFF receptor (BAFF R, also known as BR3 or TNFSFR13C), which is the principal BAFF receptor (6‑8). All three receptors are type III transmembrane proteins that are expressd in B cells. BAFF and APRIL can form active heteromers that bind to TACI (9). BAFF is expressed in peripheral blood mononuclear cells, in spleen and lymph nodes. Its expression in resting monocytes is up-regulated by IFN-alpha, IFN-beta, LPS and IL-10. BAFF provides critical survival signals to a subset of B cells with intermediate maturation status (T2 B cells) during the immune response (10). BAFF also plays an important role in the development of lymphoid tissue and enhances the survival of activated memory B cells (7, 11). Human and mouse BAFF share 86% aa sequence identity (1).

1. Schneider, P. et al. (1999) J. Exp. Med. 189:1747.
2. Mukhopadhyay, A. et al. (1999) J. Biol. Chem. 274:15978.
3. Karpusas, M. et al. (2002) J. Mol. Biol. 315:1145.
4. Liu, Y. et al. (2002) Cell 108:383.
5. Cheema, G.S. et al. (2001) Arthr. Rheum. 44:1313.
6. Marsters, S.A. et al. (2000) Curr. Biol. 10:785.
7. Thompson, J.S. et al. (2001) Science 293:2108.
8. Ng, L.G. et al. (2004) J. Immunol. 173:807.
9. Roschke, V. et al. (2002) J. Immunol. 169:4314.
10. Batten, M. et al. (2000) J. Exp. Med. 192:1453.
11. Avery, D.T. et al. (2003) J. Clin. Invest. 112:286.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for BAFF/BLyS/TNFSF13B Antibody (AF124)(10)

Publications using AF124

• Y Cao, G Lu, X Chen, X Chen, N Guo, W Li BAFF is involved in the pathogenesis of IgA nephropathy by activating the TRAF6/NF?&amp;kappaB signaling pathway in glomerular mesangial cells Mol Med Rep, 2020;21(2):795-805. 2020 [PMID: 31974601] (IHC, Human)
• M Imbrechts, K De Samblan, K Fierens, E Brisse, J Vandenhaut, T Mitera, C Libert, I Smets, A Goris, C Wouters, P Matthys IFN-? stimulates CpG-induced IL-10 production in B cells via p38 and JNK signalling pathways Eur. J. Immunol., 2018;0(0):. 2018 [PMID: 30004580] (Neutralization, Human)
• O Berggren, N Hagberg, A Alexsson, G Weber, L Rönnblom, ML Eloranta Plasmacytoid dendritic cells and RNA-containing immune complexes drive expansion of peripheral B cell subsets with an SLE-like phenotype PLoS ONE, 2017;12(8):e0183946. 2017 [PMID: 28846748] (Neutralization, Human)
• Hamada T, Samukawa T, Kumamoto T, Hatanaka K, Tsukuya G, Yamamoto M, Machida K, Watanabe M, Mizuno K, Higashimoto I, Inoue Y, Inoue H Serum B cell-activating factor (BAFF) level in connective tissue disease associated interstitial lung disease. BMC Pulm Med, 2015;15(0):110. 2015 [PMID: 26424433] (IHC-P, Human)
• Abe T, AlSarhan M, Benakanakere M, Maekawa T, Kinane D, Cancro M, Korostoff J, Hajishengallis G The B Cell-Stimulatory Cytokines BLyS and APRIL Are Elevated in Human Periodontitis and Are Required for B Cell-Dependent Bone Loss in Experimental Murine Periodontitis. J Immunol, 2015;195(4):1427-35. 2015 [PMID: 26150532] (IHC, Human)
• Saulep-Easton D, Vincent F, Quah P, Wei A, Ting S, Croce C, Tam C, Mackay F The BAFF receptor TACI controls IL-10 production by regulatory B cells and CLL B cells. Leukemia, 2016;30(1):163-72. 2016 [PMID: 26139429] (Neutralization, Human)
• Zhu J, Sun L, Lin S BlyS is up-regulated by hypoxia and promotes migration of human breast cancer cells. J. Exp. Clin. Cancer Res., 2012;31(0):31. 2012 [PMID: 22463935] (Western Blot, Human)
• Kuo SH, Yen RF, Lin CW, Chen LT, Tien HF, Cheng AL Unusual presentation of multiple pathologic bone fractures in a patient with gastric mucosa-associated lymphoid tissue lymphoma. Ann. Hematol., 2010;89(4):431-6. 2010 [PMID: 19688210] (ICC, Human)
• Bergamin F, Vincent IE, Summerfield A, McCullough KC Essential role of antigen-presenting cell-derived BAFF for antibody responses. Eur. J. Immunol., 2007;37(11):3122-30. 2007 [PMID: 17935087] (ICC, Porcine)
• Elsawa SF, Novak AJ, Grote DM, Ziesmer SC, Witzig TE, Kyle RA, Dillon SR, Harder B, Gross JA, Ansell SM B-lymphocyte stimulator (BLyS) stimulates immunoglobulin production and malignant B-cell growth in Waldenstrom macroglobulinemia. Blood, 2006;107(7):2882-8. 2006 [PMID: 16304043] (IHC-P, Human)

Bioinformatics

• Gene Symbol: TNFSF13B
• Uniprot:
  • Q9Y275()