BAFF/BLyS/TNFSF13B Antibody (H4-C7)

Images

 
Western Blot: BAFF/BLyS/TNFSF13B Antibody (H4-C7) [NBP1-04265] - Tissue lysates of Rat spleen (60ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human BLyS (1:500). Proteins were ...read more
Immunocytochemistry/ Immunofluorescence: BAFF/BLyS/TNFSF13B Antibody (H4-C7) [NBP1-04265] - ICC/IF analysis of BLyS in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human BLyS ...read more
Flow Cytometry: BAFF/BLyS/TNFSF13B Antibody (H4-C7) [NBP1-04265] - Flow cytometry analysis of BLyS in PBMC cell line, staining at 2-5ug for 1x106cells (red line). The secondary antibody used goat anti-mouse IgG Alexa ...read more

Product Details

Summary
Reactivity Hu, RtSpecies Glossary
Applications WB, ELISA, Flow, ICC/IF
Clone
H4-C7
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1.0 mg/ml

BAFF/BLyS/TNFSF13B Antibody (H4-C7) Summary

Immunogen
Recombinant human BLyS (134-285aa) purified from E. coli
Specificity
Anti-human BLyS mAb, clone H4-C7, is derived from hybridization of mouse FO myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human BLyS protein.
Isotype
IgG3 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
TNFSF13B
Purity
Protein G purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:1000
  • ELISA 1:100-1:2000
  • Flow Cytometry
  • Immunocytochemistry/Immunofluorescence 1:100

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.4) and 10% Glycerol
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein G purified

Alternate Names for BAFF/BLyS/TNFSF13B Antibody (H4-C7)

  • ApoL related ligand TALL-1
  • B lymphocyte stimulator
  • BAFF
  • BAFFB-cell activating factor
  • B-cell-activating factor
  • BLyS
  • BLYSB-lymphocyte stimulator
  • CD257 antigen
  • CD257
  • Dendritic cell-derived TNF-like molecule
  • DTL
  • TALL1
  • TALL-1delta BAFF
  • TALL1Delta4 BAFF
  • THANK
  • TNF- and APOL-related leukocyte expressed ligand 1
  • TNF and ApoL-related leukocyte expressed ligand 1
  • TNF homolog that activates apoptosis
  • TNFSF13B
  • TNFSF20
  • tumor necrosis factor (ligand) superfamily, member 13b
  • tumor necrosis factor (ligand) superfamily, member 20
  • tumor necrosis factor ligand superfamily member 13B
  • tumor necrosis factor superfamily, member 13b
  • tumor necrosis factor-like protein ZTNF4
  • ZTNF4

Background

B lymphocyte stimulator (BLyS),a member of the tumor necrosis factor (TNF) superfamily of cytokines, induces B-cell proliferation and immunoglobulin secretion and is a key regulator of peripheral B-cell populations in vivo.. BLyS is made by immune-cells called monocytes and macrophages. When monocytes and macrophages are activated, BLyS is released and binds to a receptor found only on B cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol TNFSF13B
Entrez
Uniprot