BAFF/BLyS/TNFSF13B Antibody (5A8) - BSA Free

Flow Cytometry: BAFF/BLyS/TNFSF13B Antibody (5A8) [NBP1-97639] - HEK 293T cells were mock transfected (thin line) or transfected with an expression plasmid enabling surface expression of the extracellular domain of mouse BAFF (thick line). Cells (5 x 10^5) were incubated on ice for 30 min in 50ul FACS buffer (PBS, 5% fetal calf serum, 0.02% sodium azide) containing 1ug/ml of MAb to BAFF (mouse) (5A8). After washing in FACS buffer, R-PE-conjugated antibody to rat IgG was added. Cells were incubated on ice for 30 min, washed and then analyzed by flow cytometry.

• Reactivity: Mu
• Applications: WB, ELISA, Flow
• Clone: 5A8
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

BAFF/BLyS/TNFSF13B Antibody (5A8) - BSA Free Summary

• Description: Novus Biologicals Rat BAFF/BLyS/TNFSF13B Antibody (5A8) - BSA Free (NBP1-97639) is a monoclonal antibody validated for use in WB, ELISA and Flow. Anti-BAFF/BLyS/TNFSF13B Antibody: Cited in 2 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Rat RBL cells expressing mouse BAFF.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Rat
• Gene: TNFSF13B
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Flow Cytometry 1:10-1:1000
  • Western Blot 1:100-1:2000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Protein G purified

Alternate Names for BAFF/BLyS/TNFSF13B Antibody (5A8) - BSA Free

• ApoL related ligand TALL-1
• B lymphocyte stimulator
• BAFF
• BAFFB-cell activating factor
• B-cell-activating factor
• BLyS
• BLYSB-lymphocyte stimulator
• CD257 antigen
• CD257
• Dendritic cell-derived TNF-like molecule
• DTL
• TALL1
• TALL-1delta BAFF
• TALL1Delta4 BAFF
• THANK
• TNF- and APOL-related leukocyte expressed ligand 1
• TNF and ApoL-related leukocyte expressed ligand 1
• TNF homolog that activates apoptosis
• TNFSF13B
• TNFSF20
• tumor necrosis factor (ligand) superfamily, member 13b
• tumor necrosis factor (ligand) superfamily, member 20
• tumor necrosis factor ligand superfamily member 13B
• tumor necrosis factor superfamily, member 13b
• tumor necrosis factor-like protein ZTNF4
• ZTNF4

Background

TNFSF13B recognizes membrane bound and soluble human and mouse(weak) BAFF.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for BAFF/BLyS/TNFSF13B Antibody (NBP1-97639)(2)

Publications using NBP1-97639

• Hashiguchi M, Kashiwakura Y, Kanno Y et al. Tumor necrosis factor superfamily member (TNFSF) 13 (APRIL) and TNFSF13B (BAFF) downregulate homeostatic immunoglobulin production in the intestines.. Cell. Immunol. 2017-10-27 [PMID: 29100594] (ELISA, Mouse)
• Batten M, Fletcher C, Ng LG et al. TNF deficiency fails to protect BAFF transgenic mice against autoimmunity and reveals a predisposition to B cell lymphoma. J Immunol. 2004-01-01 [PMID: 14707051]

Bioinformatics

• Gene Symbol: TNFSF13B