BAFF/BLyS/TNFSF13B Antibody (148725) [Unconjugated]

Recombinant Human BAFF/BLyS/TNFSF13B (Catalog # 2149-BF) stimulates proliferation in mouse B cells in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human BAFF/BLyS/TNFSF13B (5 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human BAFF/BLyS/TNFSF13B Monoclonal Antibody (Catalog # MAB124). At 20 µg/mL, this anti-BAFF/BLyS/TNFSF13B antibody will neutralize >60% of Recombinant Human BAFF/BLyS/TNFSF13B-induced proliferation of mouse B cells.

• Reactivity: Hu
• Applications: B/N
• Clone: 148725
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

BAFF/BLyS/TNFSF13B Antibody (148725) [Unconjugated] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human BAFF
  Ala134-Leu285
  Accession # Q9Y275
• Specificity: Detects human BAFF in direct ELISAs.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: TNFSF13B
• Endotoxin Note: <0.10 EU per 1 μg of the antibody by the LAL method.

Applications/Dilutions

• Dilutions:
  • Neutralization 20 ug/mL
• Control:

  BAFF/BLyS/TNFSF13B Knockout A549 Cell Lysate

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for BAFF/BLyS/TNFSF13B Antibody (148725) [Unconjugated]

• ApoL related ligand TALL-1
• B lymphocyte stimulator
• BAFF
• BAFFB-cell activating factor
• B-cell-activating factor
• BLyS
• BLYSB-lymphocyte stimulator
• CD257 antigen
• CD257
• Dendritic cell-derived TNF-like molecule
• DTL
• TALL1
• TALL-1delta BAFF
• TALL1Delta4 BAFF
• THANK
• TNF- and APOL-related leukocyte expressed ligand 1
• TNF and ApoL-related leukocyte expressed ligand 1
• TNF homolog that activates apoptosis
• TNFSF13B
• TNFSF20
• tumor necrosis factor (ligand) superfamily, member 13b
• tumor necrosis factor (ligand) superfamily, member 20
• tumor necrosis factor ligand superfamily member 13B
• tumor necrosis factor superfamily, member 13b
• tumor necrosis factor-like protein ZTNF4
• ZTNF4

Background

BAFF, also known as B-lymphocyte stimulator (BLyS), TNF- and APOL-related leukocyte expressed ligand 1 (TALL-1), and TNF homolog that activates apoptosis, NFKB and JNK (THANK), is a TNF ligand superfamily member and has been designated TNFSF13B.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for BAFF/BLyS/TNFSF13B Antibody (MAB124)(5)

Publications using MAB124

• H Touil, A Kobert, N Lebeurrier, A Rieger, P Saikali, C Lambert, L Fawaz, CS Moore, A Prat, J Gommerman, JP Antel, Y Itoyama, I Nakashima, A Bar-Or, Human central nervous system astrocytes support survival and activation of B cells: implications for MS pathogenesis J Neuroinflammation, 2018;15(1):114. 2018 [PMID: 29673365] (Neutralization, Human)
• Endotoxemia contributes to CD27+ memory B-cell apoptosis via enhanced sensitivity to Fas ligation in patients with Cirrhosis Sci Rep, 2016;6(0):36862. 2016 [PMID: 27857173] (Neutralization, Human)
• Giacomini E, Severa M, Rizzo F, Mechelli R, Annibali V, Ristori G, Riccieri V, Salvetti M, Coccia E IFN-beta therapy modulates B-cell and monocyte crosstalk via TLR7 in multiple sclerosis patients. Eur J Immunol, 2013;43(7):1963-72. 2013 [PMID: 23636665] (Neutralization, Human)
• Benito-Miguel M, Garcia-Carmona Y, Balsa A, Bautista-Caro MB, Arroyo-Villa I, Cobo-Ibanez T, Bonilla-Hernan MG, De Ayala CP, Sanchez-Mateos P, Martin-Mola E, Miranda-Carus ME IL-15 Expression on RA Synovial Fibroblasts Promotes B Cell Survival. PLoS ONE, 2012;7(7):e40620. 2012 [PMID: 22792388] (Flow Cytometry, Human)
• Fu L, Lin-Lee YC, Pham LV, Tamayo A, Yoshimura L, Ford RJ Constitutive NF-kappaB and NFAT activation leads to stimulation of the BLyS survival pathway in aggressive B-cell lymphomas. Blood, 2006;107(11):4540-8. 2006 [PMID: 16497967] (ICC, Human)

Bioinformatics

• Gene Symbol: TNFSF13B
• Uniprot:
  • Q9Y275()