BAF53A Antibody (3C4) - Azide and BSA Free

Western Blot: BAF53A Antibody (3C4) [H00000086-M10] - Analysis of ACTL6A expression in transfected 293T cell line by ACTL6A monoclonal antibody (M10), clone 3C4. Lane 1: ACTL6A transfected lysatE (47.5 KDa). Lane 2: Non-transfected lysate.

ELISA: BAF53A Antibody (3C4) [H00000086-M10] - Detection limit for recombinant GST tagged ACTL6A is approximately 0.3ng/ml as a capture antibody.

• Reactivity: Hu
• Applications: WB, ELISA
• Clone: 3C4
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

BAF53A Antibody (3C4) - Azide and BSA Free Summary

• Description: Quality control test: Antibody Reactive Against Recombinant Protein.
• Immunogen: ACTL6A (NP_817126, 1 a.a. ~ 80 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MVVERDDGSTLMEIDGDKGKQGGPTYYIDTNALRVPRENMEAISPLKNGMVEDWDSFQAILDHTYKMHVKSEASLHPVLM
• Specificity: ACTL6A - actin-like 6A (3C4)
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: ACTL6A
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Western Blot 1:500
• Application Notes: Antibody reactivity against Recombinant Protein with GST tag on ELISA and WB. GST tag alone is used as a negative control.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for BAF53A Antibody (3C4) - Azide and BSA Free

• actin-like 6A
• actin-like protein 6A
• actin-related protein 4,53 kDa BRG1-associated factor A
• Actin-related protein Baf53a
• Actl6
• Arp4
• arpNbeta
• BAF complex 53 kDa subunit
• Baf53a
• BAF53AACTL6
• BAF53MGC5382
• BRG1-associated factor 53A
• hArpN beta
• INO80 complex subunit K
• INO80KARPN-BETA

Background

This gene encodes a family member of actin-related proteins (ARPs), which share significant amino acid sequence identity to conventional actins. Both actins and ARPs have an actin fold, which is an ATP-binding cleft, as a common feature. The ARPs are involved in diverse cellular processes, including vesicular transport, spindle orientation, nuclear migration and chromatin remodeling. This gene encodes a 53 kDa subunit protein of the BAF (BRG1/brm-associated factor) complex in mammals, which is functionally related to SWI/SNF complex in S. cerevisiae and Drosophila; the latter is thought to facilitate transcriptional activation of specific genes by antagonizing chromatin-mediated transcriptional repression. Together with beta-actin, it is required for maximal ATPase activity of BRG1, and for the association of the BAF complex with chromatin/matrix. Three transcript variants that encode two different protein isoforms have been described.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ACTL6A
• Entrez:
  • 86(Human)
• Uniprot:
  • NP_817126(Human)