BACE-1 Antibody

Images

 
Western Blot: BACE-1 Antibody [NBP1-77307] - Analysis of BACE in human brain tissue lysate in the absence (A) or presence (B) of blocking peptide (2253P) and in mouse 3T3 cell lysate (C) with anti-BACE (CT) using ...read more
Immunocytochemistry/ Immunofluorescence: BACE-1 Antibody [NBP1-77307] - Staining of BACE in 3T3 cells with BACE antibody at 20 ug/ml.
Immunohistochemistry: BACE-1 Antibody [NBP1-77307] - Staining of mouse brain tissue with antibody at 2.5 ug/ml.
Immunocytochemistry/ Immunofluorescence: BACE-1 Antibody [NBP1-77307] - 3T3 cells with BACE1 antibody at 10 ug/ml.

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

BACE-1 Antibody Summary

Immunogen
Antibody was raised against a peptide corresponding to 17 amino acids at the carboxy terminus of human BACE. The immunogen is located within the last 50 amino acids of BACE.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
BACE1
Purity
Ion exchange chromatography
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.5-1 ug/ml
  • ELISA 1:100-1:2000
  • Immunocytochemistry/Immunofluorescence 10 ug/ml
  • Immunohistochemistry 2.5 ug/ml
  • Immunohistochemistry-Paraffin 2.5 ug/ml
Theoretical MW
70 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control Peptide
BACE-1 Peptide (NBP1-77307PEP)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Ion exchange chromatography

Alternate Names for BACE-1 Antibody

  • APP beta-secretase
  • ASP2
  • Aspartyl protease 2
  • BACE1
  • BACE-1
  • BACEAsp 2
  • beta-secretase 1 precursor variant 1
  • beta-secretase 1
  • beta-site amyloid beta A4 precursor protein-cleaving enzyme
  • Beta-site amyloid precursor protein cleaving enzyme 1
  • Beta-site APP cleaving enzyme 1
  • beta-site APP-cleaving enzyme 1
  • beta-site APP-cleaving enzyme
  • EC 3.4.23
  • EC 3.4.23.46
  • FLJ90568
  • HSPC104
  • KIAA1149
  • memapsin-2
  • Membrane-associated aspartic protease 2
  • transmembrane aspartic proteinase Asp2

Background

Accumulation of the amyloid-beta (Abeta) plaque in the cerebral cortex is a critical event in the pathogenesis of Alzheimer's disease. Abeta peptide is generated by proteolytic cleavage of the beta-amyloid protein precursor (APP) at beta- and gamma-sites by two proteases. APP is first cleaved by beta-secretase, producing a soluble derivative of the protein and a membrane anchored 99-amino acid carboxy-terminal fragment (C99). The C99 fragment serves as substrate for gamma-secretase to generate the 4 kDa amyloid-beta peptide, which is deposited in the brains of all suffers of Alzheimer's disease. The long-sought beta-secretase was recently identified by several groups independently and designated beta-site APP cleaving enzyme (BACE) and aspartyl protease 2 (Asp2). BACE/Asp2 is a novel transmembrane aspartic protease and colocalizes with APP.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol BACE1
Entrez
Uniprot