B4GALNT1 Antibody (1024936) [Unconjugated]

GM2/GD2 Synthase/ B4GALNT1 was detected in immersion fixed U‑251 MG human glioblastoma cell line using Mouse Anti-Human GM2/GD2 Synthase/ B4GALNT1 Monoclonal Antibody (Catalog # MAB105031) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. Staining was performed using our protocol for Fluorescent ICC Staining of Non-adherent Cells.

• Reactivity: Hu
• Applications: ICC/IF
• Clone: 1024936
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

B4GALNT1 Antibody (1024936) [Unconjugated] Summary

• Immunogen: S. frugiperda insect ovarian cell line Sf 21-derived recombinant human GM2/GD2 Synthase/ B4GALNT1
  Ser26-Gln533
  Accession # Q00973
• Specificity: Detects human GM2/GD2 Synthase/ B4GALNT1in direct ELISAs.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry 8-25 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for B4GALNT1 Antibody (1024936) [Unconjugated]

• (N-acetylneuraminyl)-galactosylglucosylceramide
• B4GALNT1
• beta-1,4 N-acetylgalactosaminyltransferase 1
• beta1,4GalNAc-T
• beta-1,4-N-acetyl-galactosaminyl transferase 1
• beta1-4GalNAc-T
• GALGT
• GALGTEC 2.4.1.92
• GALNACT
• GalNAc-T
• GD2 Synthase
• GD2 synthase, GM2 synthase
• GM2 Synthase
• GM2/GD2 synthase
• SIAT2
• SPG26
• UDP-Gal:betaGlcNAc beta-1,4-N-acetylgalactosaminyltransferase transferase 1
• UDP-N-acetyl-alpha-D-galactosamine:(N-acetylneuraminyl)-galactosylglucosylceramideN-acetylgalactosaminyltransferase (GalNAc-T)

Background

B4GALNT1, also known as GM2/GD2 Synthase, is an enzyme involved in the biosynthesis of brain-enriched complex gangliosides GM2, GD2, GT2 and GA2 from GM3, GD3, GT3 and GA3.  This is a critical step in the synthesis of all complex gangliosides enriched in the nervous system of vertebrates.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Uniprot:
  • Q00973()