![Western Blot: Axin-1 Antibody [NBP3-18243] - Western blot analysis of Rat Kidney showing detection of ~95 kDa Axin-1 protein using Rabbit Anti-Axin-1 Polyclonal Antibody (NBP3-18243). Lane 1: Molecular Weight Ladder (MW). Lane 2: Rat Kidney. Load: 15 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-Axin-1 Polyclonal Antibody (NBP3-18243) at 1:1000 for 16 hours at 4C. Secondary Antibody: Goat Anti-Rabbit IgG: HRP at 1:3000 for 1 hour at RT. Color Development: ECL solution for 5 min at RT. Predicted/Observed Size: ~95 kDa.](http://images.novusbio.com/fullsize/Axin-1 Antibody-Western Blot-NBP3-18243-img0003.jpg "Western Blot: Axin-1 Antibody [NBP3-18243] - Western blot analysis of Rat Kidney showing detection of ~95 kDa Axin-1 protein using Rabbit Anti-Axin-1 Polyclonal Antibody (NBP3-18243). Lane 1: Molecular Weight Ladder (MW). Lane 2: Rat Kidney. Load: 15 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-Axin-1 Polyclonal Antibody (NBP3-18243) at 1:1000 for 16 hours at 4C. Secondary Antibody: Goat Anti-Rabbit IgG: HRP at 1:3000 for 1 hour at RT. Color Development: ECL solution for 5 min at RT. Predicted/Observed Size: ~95 kDa.")
| Reactivity | Hu, RtSpecies Glossary |
| Applications | WB, IHC |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Concentration | 1 mg/ml |
| Immunogen | Synthetic peptide from the mid-protein of Human Axin-1 (aa. 470-570) |
| Localization | Cytoplasm|Nucleus|Membrane|Cell Membrane |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | AXIN1 |
| Purity | Peptide affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | Optimal dilutions for assays should be determined by the user. |
| Storage | Store at -20C. Avoid freeze-thaw cycles. |
| Buffer | PBS pH 7.4, 50% glycerol |
| Preservative | 0.09% Sodium Azide |
| Concentration | 1 mg/ml |
| Purity | Peptide affinity purified |
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Secondary Antibodies |
Isotype Controls |
Research Areas for Axin-1 Antibody (NBP3-18243)Find related products by research area.
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The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | AXIN1 |
![Immunohistochemistry: Axin-1 Antibody [NBP3-18243] - Immunohistochemistry analysis using Rabbit Anti-Axin 1 Polyclonal Antibody (NBP3-18243). Tissue: Breast. Species: Human. Fixation: Formalin Fixed Paraffin-Embedded. Primary Antibody: Rabbit Anti-Axin 1 Polyclonal Antibody (NBP3-18243) at 1:50 for 30 min at RT. Counterstain: Hematoxylin. Magnification: 10X. HRP-DAB Detection.](http://images.novusbio.com/fullsize/Axin-1-Antibody-Immunohistochemistry-NBP3-18243-img0002.jpg "Immunohistochemistry: Axin-1 Antibody [NBP3-18243] - Immunohistochemistry analysis using Rabbit Anti-Axin 1 Polyclonal Antibody (NBP3-18243). Tissue: Breast. Species: Human. Fixation: Formalin Fixed Paraffin-Embedded. Primary Antibody: Rabbit Anti-Axin 1 Polyclonal Antibody (NBP3-18243) at 1:50 for 30 min at RT. Counterstain: Hematoxylin. Magnification: 10X. HRP-DAB Detection.")
![Immunohistochemistry: Axin-1 Antibody [NBP3-18243] - Immunohistochemistry analysis using Rabbit Anti-Axin 1 Polyclonal Antibody (NBP3-18243). Tissue: Breast. Species: Human. Fixation: Formalin Fixed Paraffin-Embedded. Primary Antibody: Rabbit Anti-Axin 1 Polyclonal Antibody (NBP3-18243) at 1:50 for 30 min at RT. Counterstain: Hematoxylin. Magnification: 10X. HRP-DAB Detection.](http://images.novusbio.com/fullsize/Axin-1-Antibody-Immunohistochemistry-NBP3-18243-img0001.jpg "Immunohistochemistry: Axin-1 Antibody [NBP3-18243] - Immunohistochemistry analysis using Rabbit Anti-Axin 1 Polyclonal Antibody (NBP3-18243). Tissue: Breast. Species: Human. Fixation: Formalin Fixed Paraffin-Embedded. Primary Antibody: Rabbit Anti-Axin 1 Polyclonal Antibody (NBP3-18243) at 1:50 for 30 min at RT. Counterstain: Hematoxylin. Magnification: 10X. HRP-DAB Detection.")
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![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")