Attractin Antibody

Immunohistochemistry-Paraffin: Attractin Antibody [NB100-58757] - ATRN / Attractin antibody (20 ug/ml) was used to stain formalin-fixed, paraffin-embedded human spleen, followed by biotinylated goat anti-rabbit IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.

Product Discontinued

Attractin Antibody Summary

• Immunogen: Synthetic 16 amino acid peptide from internal region of human ATRN / Attractin.
• Epitope: internal domain of human protein
• Specificity: Human Attractin. BLAST analysis of the peptide immunogen showed no homology with other human proteins, except KCTD19 (50%).
• Predicted Species: Primate (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: ATRN
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 20 ug/ml
• Application Notes: IHC: NB100-58757 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity.

Reactivity Notes

Predicted cross-reactivity based on sequence identity: Gorilla (100%), Gibbon (94%), Monkey (88%), Bovine (88%), Porcine (88%), Marmoset (81%), Rat (81%), Bat (81%), Hamster (81%).

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.1% Sodium Azide
• Concentration: 0.7 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Attractin Antibody

• ATRN
• Attractin
• DPPT-L
• KIAA0548
• Mahogany Protein
• MGC126754
• MGCA

Background

Attractin is a serum glycoprotein of 175 kDaand found in both membrane-bound and secreted forms as a result of alternative splicing. Both the secreted and membrane-bound forms of attractin may be involved in the development and maintenance of the central nervous system. Membrane-bound attractin is a co-receptor for Agouti, antagonist of melanocortin-1 receptor. Secreted attractin, expressed by activated T lymphocytes and modulates interactions between T cells and monocytes/macrophages, was examined as a potential marker of immune activity. Attractinmay be a component of a pathway for regulated protein turnover that also involves mahogunin, a wide-expressed E3 ubiquitinligase found at particularly high levels in the brain. Attractinwas considered as an extracellular target amenable for the development of obesity-regulating drugs, also.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ATRN
• Entrez:
  • 8455(Human)