Atrial Natriuretic Peptide/ANP Antibody

Images

 
Western Blot: Atrial Natriuretic Peptide/ANP Antibody [NBP1-97752] - 0.01ug/ml Analysis of Mouse Heart lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Immunohistochemistry-Paraffin: Atrial Natriuretic Peptide/ANP Antibody [NBP1-97752] - Staining of paraffin embedded Human Heart. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, IHC, IHC-P, PEP-ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

Atrial Natriuretic Peptide/ANP Antibody Summary

Immunogen
Peptide with sequence RIGAQSGLGCNSFR corresponding to C-Terminus according to NP_006163.1.
Specificity
The immunizing peptide represents aa137-150 of the precursor NPPA.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
NPPA
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.01 - 0.03 ug/ml
  • Immunohistochemistry 5 - 10 ug/ml
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Peptide ELISA Detection limit 1:32000
Application Notes
WB: Approx. 19 kDa band observed in mouse and rat heart lysates (calculated MW of 16.4 kDa band according to human precursor NP_006163.1 and 16.6 kDa band accordiong to mouse precursor NP_032751.1). IHC-P: Human heart shows vesicular staining inside the cardiac fibrils, accumulating towards the cell membrane.
Publications
Read Publication using NBP1-97752.

Reactivity Notes

Predicted cross-reactivity based on sequence identity: Canine, Porcine, Bovine.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Tris (pH 7.3), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Atrial Natriuretic Peptide/ANP Antibody

  • ANF
  • ANP
  • ANPatriopeptin
  • ATFB6
  • atrial natriuretic factor
  • Atrial Natriuretic Peptide
  • Cardionatrin
  • CDD-ANF
  • natriuretic peptide A
  • natriuretic peptide precursor A
  • NPPA
  • PND
  • PNDcardionatrin
  • prepronatriodilatin

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Atrial Natriuretic Peptide/ANP Antibody (NBP1-97752)(1)

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WB Video Protocol

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol NPPA
Entrez
Uniprot