Atrial Natriuretic Peptide/ANP Antibody

Western Blot: Atrial Natriuretic Peptide/ANP Antibody [NBP1-97752] - Analysis of Mouse Heart lysate (35 ug protein in RIPA buffer). Antibody at 0.01 ug/mL. Primary incubation was 1 hour. Detected by chemiluminescence.

Immunohistochemistry-Paraffin: Atrial Natriuretic Peptide/ANP Antibody [NBP1-97752] - Staining of paraffin embedded Human Heart. Antibody at 5 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

• Reactivity: Hu, Mu, Rt, Po, Bv, Ca
• Applications: WB, ELISA, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

Atrial Natriuretic Peptide/ANP Antibody Summary

• Description: Novus Biologicals Goat Atrial Natriuretic Peptide/ANP Antibody (NBP1-97752) is a polyclonal antibody validated for use in IHC, WB, ELISA and ICC/IF. Anti-Atrial Natriuretic Peptide/ANP Antibody: Cited in 2 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Peptide with sequence RIGAQSGLGCNSFR corresponding to C-Terminus according to NP_006163.1.
• Specificity: The immunizing peptide represents aa137-150 of the precursor NPPA.
• Predicted Species: Porcine (100%), Bovine (100%), Canine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: NPPA
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 5 - 10 ug/mL
  • Immunohistochemistry-Paraffin 5 - 10 ug/mL
  • Peptide ELISA Detection limit 1:32000
  • Western Blot 0.01 - 0.03 ug/mL
• Application Notes: WB: Approx. 19 kDa band observed in mouse and rat heart lysates (calculated MW of 16.4 kDa band according to human precursor NP_006163.1 and 16.6 kDa band accordiong to mouse precursor NP_032751.1). IHC-P: Human heart shows vesicular staining inside the cardiac fibrils, accumulating towards the cell membrane.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Atrial Natriuretic Peptide/ANP Antibody

• ANF
• ANP
• ANPatriopeptin
• ATFB6
• atrial natriuretic factor
• Atrial Natriuretic Peptide
• Cardionatrin
• CDD-ANF
• natriuretic peptide A
• natriuretic peptide precursor A
• NPPA
• PND
• PNDcardionatrin
• prepronatriodilatin

Background

Atrial natriuretic factor (ANP) is a potent vasoactive substance which is thought to play a key role in cardiovascular homeostasis and has a cGMP stimulating activity. There are two different prepronatriodilatin alleles. One codes for 2 Arginine residues at the C terminus that are cleaved to form the mature peptide, while the other ends in a termination codon immediately after the last codon of the mature peptide. ANP belongs to the natriuretic peptide family.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Atrial Natriuretic Peptide/ANP Antibody (NBP1-97752)(2)

Publications using NBP1-97752

• Al-Qazazi R, Lima PDA, Prisco SZ Et al. Macrophage-NLRP3 Activation Promotes Right Ventricle Failure in Pulmonary Arterial Hypertension Am J Respir Crit Care Med 2022-06-14 [PMID: 35699679] (ICC/IF, Rat)
  
  Details:
  Citation using the Alexa Fluor 700 version of this antibody.
• Ibebuogu UN, Gladysheva IP, Houng AK, Reed GL. Decompensated heart failure is associated with reduced corin levels and decreased cleavage of pro-atrial natriuretic peptide. Circ Heart Fail 2011-03-01 [PMID: 21216831]

Bioinformatics

• Gene Symbol: NPPA
• Entrez:
  • 4878(Human)
  • 230899(Mouse)
  • 24602(Rat)
• Uniprot:
  • NP_006163.1(Human)