ATPase Activity Kit (Colorimetric)

Images

 
There are currently no images for ATPase Kit (601-0120).

Every product we sell is backed by Novus' 100% Guarantee. If you have used this product, please submit your images and reviews to earn reward points.

Product Details

Summary
Applications In vitro

Order Details

ATPase Activity Kit (Colorimetric) Summary

Description
This non radioactive colorimetric assay kit contains all the necessary reagents for measuring enzyme activity (everything included in the PiColorlock kit and more) and is ideal for high throughput drug screening.

The kits contain PiColorlock, a non-radioactive, superior phosphate detection reagent.

Key Features of PiColorLock:
  • Colorimetric Assay-competitor assays are radioactive.
  • Special additive ensures low backgrounds with acid-labile substrates.
  • Unparalleled stability of phosphate-dye complexes.
  • Reagent is compatible with almost any assay buffer.
  • No inhibition of color development by high concentrations of protein.
  • Stable reagent formulation - long shelf life.
Colorimetric assays for ATPases are invariably based on the formation of colored complexes between an inorganic phosphate and a dye molecule under acidic conditions.
Kit Type
Activity Kit (Colorimetric)
Gene
DNAH8

Applications/Dilutions

Application Notes
Na+/K+-ATPase activity determination (PMID: 21304972)
Reviewed Applications
Read 1 Review rated 4
using
601-0120 in the following application:

Publications
Read Publications using
601-0120 in the following applications:

Packaging, Storage & Formulations

Storage
Storage of components varies. See protocol for specific instructions.

Kit Components

Components
  1. 1 x 10 ml of PiColorLock (1 x 25 ml)
  2. 1 x 0.25 ml of Accelerator (1 x 0.5 ml)
  3. 1 x 5 ml of Stabiliser (1 x 10 ml)
  4. 1 x 1.5 ml of 0.1M MgCl2 (2 x 1.5 ml)
  5. 1 x 5 ml of 0.5M Tris pH 7.5 (1 x 10 ml)
  6. 1 x 5 ml of 0.1mM Pi standard (1 x 10 ml)
  7. 4 vials of lyophilized ATP (10 vials)
  8. 2 x 96-well plates (5 plates)

Notes

This product is manufactured by Expedeon Inc. and distributed by Novus Biologicals.

Alternate Names for ATPase Activity Kit (Colorimetric)

  • ATPase
  • axonemal
  • dynein, axonemal, heavy chain 8
  • dynein, axonemal, heavy polypeptide 8
  • FLJ25850
  • FLJ36115
  • FLJ36334
  • hdhc9

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Kits are guaranteed for 6 months from date of receipt.

Customers Who Viewed This Item Also Viewed...

NBP1-19015
Species: Hu, Mu
Applications: WB, Simple Western, ELISA, ICC/IF, IP
NBP2-02000
Species: Hu
Applications: WB, Flow, ICC/IF
NBP2-30949
Species: Hu
Applications: IHC, IHC-P
NBP1-97308
Species: Hu, Mu, Rt
Applications: WB, Simple Western, DB, ICC/IF, IHC, IHC-P
AF5055
Species: Hu
Applications: WB
NBP2-29463
Species: Hu, Mu, Rt, Pm
Applications: WB, Flow, ICC/IF, IHC, IHC-Fr, IHC-P
AF8691
Species: Hu, Mu, Rt
Applications: WB, Simple Western, IHC, KO
NBP2-31366
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
NBP1-81734
Species: Hu, Mu, Rt, Dr
Applications: WB, Simple Western, ICC/IF, IHC, IHC-P, KD
NBP1-81293
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P, KD
AF1519
Species: Hu
Applications: WB, IHC
NBP2-32432
Species: Hu
Applications: ICC/IF, IHC, IHC-P
NBP1-92041
Species: Hu
Applications: WB, IHC, IHC-P
NBP2-67484
Species: Hu, Mu, Rt
Applications: WB, Flow, ICC/IF, IHC, IHC-P, IP
NBP1-97512
Species: Mu
Applications: WB, ICC/IF, IHC, IHC-Fr, IHC-P, IP
NBP1-59448
Species: Hu, Mu, Rt, Bv, Ca, Eq, GP, Rb
Applications: WB
NBP2-67901
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
NBP1-77461
Species: Hu, Mu
Applications: WB, ICC/IF, IHC, IHC-P
AF4499
Species: Hu, Mu, Rt
Applications: WB, Simple Western
601-0120
Applications: In vitro

Publications for ATPase Kit (601-0120)(73)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 1 application: Elisa.


Filter By Application
Elisa
(1)
All Applications
Filter By Species
Mouse
(1)
All Species
Showing Publications 1 - 10 of 73. Show All 73 Publications.
Publications using 601-0120 Applications Species
Zadorozhny K, Sannino V, Belan O et al. Fanconi-Anemia-Associated Mutations Destabilize RAD51 Filaments and Impair Replication Fork Protection. Cell Rep. 2017 Oct 10 [PMID: 29020621]
Granja AG, Tafalla C. Different IgM+ B cell subpopulations residing within the peritoneal cavity of vaccinated rainbow trout are differently regulated by BAFF. Fish Shellfish Immunol. 2017 Oct 5 [PMID: 28989090]
Park SA, Hong BZ, Ha KC et al. Protein tyrosine phosphatase 1B is a mediator of cyclic ADP ribose-induced Ca2+ signaling in ventricular myocytes. Exp Mol Med. 2017 Jun 2 [PMID: 28572573]
Bittencourt IA, Serpeloni M, Hiraiwa PM et al. Dissecting biochemical peculiarities of the ATPase activity of TcSub2, a component of the mRNA export pathway in Trypanosoma cruzi. Int J Biol Macromol. 2017 [PMID: 28212935]
Huerta-Uribe A, Marjenberg ZR, Yamaguchi N et al. Identification and Characterization of Novel Compounds Blocking Shiga Toxin Expression in Escherichia coli O157:H7. Front Microbiol. 2016 Nov 30 [PMID: 27965652]
Martino L, Holland L, Christodoulou E et al. The Biophysical Characterisation and SAXS Analysis of Human NLRP1 Uncover a New Level of Complexity of NLR Proteins. PLoS One. 2016 Oct 11 [PMID: 27727326]
Pelkonen L, Reinisalo M, Morin-Picardat E et al. Isolation of Intact and Functional Melanosomes from the Retinal Pigment Epithelium. PLoS One 2016 Aug 23 [PMID: 27551967]
Sheng Y, Yang X, Lian Y et al. Characterization of a cadmium resistance Lactococcus lactissubsp. lactis strain by antioxidant assays and proteome profiles methods. Environ Toxicol Pharmacol. 2016 [PMID: 27522548]
Kristensen ML, Kierulf-Lassen C, Nielsen PM et al. Remote ischemic perconditioning attenuates ischemia/reperfusion-induced downregulation of AQP2 in rat kidney. Physiol Rep. 2016 [PMID: 27405971]
Wambach JA, Yang P, Wegner DJ et al. Functional Characterization of ABCA3 Mutations from Infants with Respiratory Distress Syndrome. Am J Respir Cell Mol Biol. 2016 [PMID: 27374344]
Show All 73 Publications.

Review for ATPase Kit (601-0120) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Mouse.

Reviews using 601-0120:
Filter by Applications
ELISA
(1)
All Applications
Filter by Species
Mouse
(1)
All Species
Images Ratings Applications Species Date Details
 
reviewed by:
Anonymous
ELISA Mouse 09/28/2011
View

Summary

ApplicationELISA
Sample TestedMouse Brain Cytoskeletal Proteins
SpeciesMouse
CommentsI was very happy to use the kit for ATPase assay. Simple and easy to perform.
Pub Med ID21359212

Comments

CommentsI was very happy to use the kit for ATPase assay. Simple and easy to perform.

FAQs for ATPase Kit (601-0120). (Showing 1 - 7 of 7 FAQs).

  1. I have phosphate in my enzyme. What can I do?
    • You can dialyse or desalt the enzyme into a phosphate-free buffer. Alternatively, you can use a special resin (PiBind) to remove the phosphate.
  2. I have 5% DMSO in my assay. Can I use PiColorLock Gold?
    • Yes, the reagent is designed for drug screening work and other situations that require DMSO.
  3. I have a high background in my ATPase assay and I definitely do not have free phosphate in my sample
    • This is almost always due to inadequate mixing of the special stabilizer with the sample and detection reagent. Make sure the stabilizer is pipetted up and down several times to ensure thorough mixing.
  4. I would like to measure the conversion of pyrophosphate to phosphate. Can I use the PiColorLock Gold Phosphate Detection System for this purpose?
    • Yes, only the phosphate will give a signal; pyrophosphate will not.
  5. I was wondering if it is possible to buy the purified ATP that comes in the following kit separately? We bought the whole kit earlier, but we are in need for more of the purified ATP component from the kit.
    • We do have extra ATP from the kit that we can send you no problem. Please send me you original order information and I can use that to get everything processed and sent out to the correct location.
  6. I'd like to detect Na-K ATPase activity. Can the product Colorimetric ATPase Assay Kit (601-0120) detect specific Na-K ATPase activity?
    • This kit is intended for the measurement of ATPase activity, but is not specific to one particular type of activity.
  7. I would like to work with ecto-ntpdase enzymes in human plasma samples. What kind of samples can it be used with the ATPase assay kit?
    • In principle our ATPase assay kits can be used to measure any ATPase. With complex samples however (e.g. membranes, homogenates) you will need to be able to distinguish hydrolysis of ATP mediated by the enzyme of interest, and hydrolysis mediated by other ATPases. Unfortunately, we do not know whether other enzymes may be present. If other enzymes are present, you will need to find assay conditions in which your enzyme is the dominant activity (by manipulating pH, buffer composition or perhaps through using specific inhibitors of the other enzymes). Another approach that some people use is to immunoprecipitate the ATPase of interest before the assay is carried out, which effectively removes all other enzymes.

Additional ATPase Products

Bioinformatics Tool for ATPase Kit (601-0120)

Discover related pathways, diseases and genes to ATPase Kit (601-0120). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for ATPase Kit (601-0120)

Discover more about diseases related to ATPase Kit (601-0120).
 

Pathways for ATPase Kit (601-0120)

View related products by pathway.

Research Areas for ATPase Kit (601-0120)

Find related products by research area.

Blogs on ATPase

There are no specific blogs for ATPase, but you can read our latest blog posts.

Contact Information

Product PDFs

Recent Reviews

4
5
0
4
1
3
0
2
0
1
0

 
Anonymous
09/28/2011
Application: ELISA
Species: Mouse

Bioinformatics

Gene Symbol DNAH8