ATPase Activity Kit (Colorimetric)


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ATPase Activity Kit (Colorimetric) Summary

This non radioactive colorimetric assay kit contains all the necessary reagents for measuring enzyme activity (everything included in the PiColorlock kit and more) and is ideal for high throughput drug screening.

The kits contain PiColorlock, a non-radioactive, superior phosphate detection reagent.

Key Features of PiColorLock:
  • Colorimetric Assay-competitor assays are radioactive.
  • Special additive ensures low backgrounds with acid-labile substrates.
  • Unparalleled stability of phosphate-dye complexes.
  • Reagent is compatible with almost any assay buffer.
  • No inhibition of color development by high concentrations of protein.
  • Stable reagent formulation - long shelf life.
Colorimetric assays for ATPases are invariably based on the formation of colored complexes between an inorganic phosphate and a dye molecule under acidic conditions.
Kit Type
Activity Kit (Colorimetric)


  • In vitro assay
Application Notes
Na+/K+-ATPase activity determination (PMID: 21304972)
Reviewed Applications
Read 1 Review rated 4
601-0120 in the following application:

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601-0120 in the following applications:

Packaging, Storage & Formulations

Storage of components varies. See protocol for specific instructions.

Kit Components

  1. 1 x 0.5ml of Accelerator ( 1 x 0.5ml)
  2. 1 x 1.5 ml of 0.1M MgCl2 (2 x 1.5 ml)
  3. 1 x 10 ml of PiColorLock ( 1 x 10ml)
  4. 1 x 5 ml of 0.1mM Pi standard (1 x 5ml)
  5. 1 x 5ml of 0.5M Tris PH 7.5 (1x 5ml)
  6. 1 x 5ml of Stabilizer (1 x 5ml)
  7. 2 x 96-well plates
  8. 4 vials of lyophilized ATP (4 vials)


This product is manufactured by Abcam and distributed by Novus Biologicals.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt and this statement overrides any mentioned guarantee period on the limitations section of this products datasheet. Please contact with questions.

Alternate Names for ATPase Activity Kit (Colorimetric)

  • ATPase
  • axonemal
  • dynein, axonemal, heavy chain 8
  • dynein, axonemal, heavy polypeptide 8
  • FLJ25850
  • FLJ36115
  • FLJ36334
  • hdhc9


CATALYTIC ACTIVITY: ATP + H2O = ADP + phosphate. SUBCELLULAR LOCATION: Membrane; multi-pass membrane protein (By similarity). ALTERNATIVE PRODUCTS: 2 named isoforms produced by alternative splicing. SIMILARITY: Belongs to the cation transport ATPase (P-type) family. Type V subfamily.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Kits are guaranteed for 6 months from date of receipt.

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Publications for ATPase Kit (601-0120)(69)

We have publications tested in 3 confirmed species: Mouse, Bacteria, E. coli.

We have publications tested in 2 applications: ELISA, EnzAct.

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Showing Publications 1 - 10 of 69. Show All 69 Publications.
Publications using 601-0120 Applications Species
Cheng WL, Kao YH, Chao TF et al. MicroRNA-133 suppresses ZFHX3-dependent atrial remodelling and arrhythmia Elife 2019-06-01 [PMID: 31152485]
Zadorozhny K, Sannino V, Belan O et al. Fanconi-Anemia-Associated Mutations Destabilize RAD51 Filaments and Impair Replication Fork Protection. Cell Rep. 2017-10-10 [PMID: 29020621]
Granja AG, Tafalla C. Different IgM+ B cell subpopulations residing within the peritoneal cavity of vaccinated rainbow trout are differently regulated by BAFF. Fish Shellfish Immunol. 2017-10-05 [PMID: 28989090]
Park SA, Hong BZ, Ha KC et al. Protein tyrosine phosphatase 1B is a mediator of cyclic ADP ribose-induced Ca2+ signaling in ventricular myocytes. Exp Mol Med. 2017-06-02 [PMID: 28572573]
Bittencourt IA, Serpeloni M, Hiraiwa PM et al. Dissecting biochemical peculiarities of the ATPase activity of TcSub2, a component of the mRNA export pathway in Trypanosoma cruzi . Int J Biol Macromol. 2017-01-01 [PMID: 28212935]
Huerta-Uribe A, Marjenberg ZR, Yamaguchi N et al. Identification and Characterization of Novel Compounds Blocking Shiga Toxin Expression in Escherichia coli O157:H7. Front Microbiol. 2016-11-30 [PMID: 27965652]
Martino L, Holland L, Christodoulou E et al. The Biophysical Characterisation and SAXS Analysis of Human NLRP1 Uncover a New Level of Complexity of NLR Proteins. PLoS One. 2016-10-11 [PMID: 27727326]
Pelkonen L, Reinisalo M, Morin-Picardat E et al. Isolation of Intact and Functional Melanosomes from the Retinal Pigment Epithelium. PLoS One 2016-08-23 [PMID: 27551967]
Sheng Y, Yang X, Lian Y et al. Characterization of a cadmium resistance Lactococcus lactissubsp. lactis strain by antioxidant assays and proteome profiles methods. Environ Toxicol Pharmacol. 2016-01-01 [PMID: 27522548]
Kristensen ML, Kierulf-Lassen C, Nielsen PM et al. Remote ischemic perconditioning attenuates ischemia/reperfusion-induced downregulation of AQP2 in rat kidney. Physiol Rep. 2016-01-01 [PMID: 27405971]
Show All 69 Publications.

Review for ATPase Kit (601-0120) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Mouse.

Reviews using 601-0120:
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reviewed by:
Panaiyur Mohan
ELISA Mouse 09/28/2011


Sample TestedMouse Brain Cytoskeletal Proteins

FAQs for ATPase Kit (601-0120). (Showing 1 - 7 of 7 FAQs).

  1. I have phosphate in my enzyme. What can I do?
    • You can dialyse or desalt the enzyme into a phosphate-free buffer. Alternatively, you can use a special resin (PiBind) to remove the phosphate.
  2. I have 5% DMSO in my assay. Can I use PiColorLock Gold?
    • Yes, the reagent is designed for drug screening work and other situations that require DMSO.
  3. I have a high background in my ATPase assay and I definitely do not have free phosphate in my sample
    • This is almost always due to inadequate mixing of the special stabilizer with the sample and detection reagent. Make sure the stabilizer is pipetted up and down several times to ensure thorough mixing.
  4. I would like to measure the conversion of pyrophosphate to phosphate. Can I use the PiColorLock Gold Phosphate Detection System for this purpose?
    • Yes, only the phosphate will give a signal; pyrophosphate will not.
  5. I was wondering if it is possible to buy the purified ATP that comes in the following kit separately? We bought the whole kit earlier, but we are in need for more of the purified ATP component from the kit.
    • We do have extra ATP from the kit that we can send you no problem. Please send me you original order information and I can use that to get everything processed and sent out to the correct location.
  6. I'd like to detect Na-K ATPase activity. Can the product Colorimetric ATPase Assay Kit (601-0120) detect specific Na-K ATPase activity?
    • This kit is intended for the measurement of ATPase activity, but is not specific to one particular type of activity.
  7. I would like to work with ecto-ntpdase enzymes in human plasma samples. What kind of samples can it be used with the ATPase assay kit?
    • In principle our ATPase assay kits can be used to measure any ATPase. With complex samples however (e.g. membranes, homogenates) you will need to be able to distinguish hydrolysis of ATP mediated by the enzyme of interest, and hydrolysis mediated by other ATPases. Unfortunately, we do not know whether other enzymes may be present. If other enzymes are present, you will need to find assay conditions in which your enzyme is the dominant activity (by manipulating pH, buffer composition or perhaps through using specific inhibitors of the other enzymes). Another approach that some people use is to immunoprecipitate the ATPase of interest before the assay is carried out, which effectively removes all other enzymes.

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Panaiyur Mohan
Application: ELISA
Species: Mouse


Gene Symbol DNAH8