Recombinant Human ATP6V1E2 GST (N-Term) Protein

12.5% SDS-PAGE Stained with Coomassie Blue.

• Reactivity: Hu
• Applications: WB, ELISA, MA, AP

Recombinant Human ATP6V1E2 GST (N-Term) Protein Summary

• Description: A recombinant protein with GST tag at N-terminal corresponding to the amino acids 1-226 of Human ATP6V1E2
  
  

  Source: Wheat Germ (in vitro)

  Amino Acid Sequence: MALSDVDVKKQIKHMMAFIEQEANEKAEEIDAKAEEEFNIEKGRLVQTQRLKIMEYYEKKEKQIEQQKKILMSTMRNQARLKVLRARNDLISDLLSEAKLRLSRIVEDPEVYQGLLDKLVLQGLLRLLEPVMIVRCRPQDLLLVEAAVQKAIPEYMTISQKHVEVQIDKEAYLAVNAAGGVEVYSGNQRIKVSNTLESRLDLSAKQKMPEIRMALFGANTNRKFFI

• Preparation Method: in vitro wheat germ expression system
• Details of Functionality: This protein was produced in an in vitro wheat germ expression system that should preserve correct conformational folding that is necessary for biological function. While it is possible that this protein could display some level of activity, the functionality of this protein has not been explicitly measured or validated.
• Source: Wheat germ
• Protein/Peptide Type: Recombinant Protein
• Gene: ATP6V1E2
• Purity: >80% by SDS-PAGE and Coomassie blue staining

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunoaffinity Purification
  • Protein Array
  • Western Blot
• Theoretical MW: 52.5 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -80C. Avoid freeze-thaw cycles.
• Buffer: 50 mM Tris-HCl, 10 mM reduced Glutathione, pH 8.0 in the elution buffer.
• Preservative: No Preservative
• Purity: >80% by SDS-PAGE and Coomassie blue staining

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Recombinant Human ATP6V1E2 GST (N-Term) Protein

• ATP6E1V-ATPase subunit E 2
• ATP6EL2
• ATP6V1EL2
• ATPase, H+ transporting, lysosomal (vacuolar proton pump) 31kD-like 2
• ATPase, H+ transporting, lysosomal 31kDa, V1 subunit E isoform 2
• ATPase, H+ transporting, lysosomal 31kDa, V1 subunit E2
• MGC9341
• Vacuolar proton pump subunit E 2
• vacuolar-type proton-translocating ATPase subunit E1
• VMA4
• V-type proton ATPase subunit E 2

Background

Subunit of the peripheral V1 complex of vacuolar ATPase essential for assembly or catalytic function.V-ATPase is responsible for acidifying a variety of intracellular compartments in eukaryotic cells. This isoform isessential for energy coupling involved in acidification of acrosome

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Bioinformatics

• Gene Symbol: ATP6V1E2