ATP6V1B2 Antibody

Western Blot: ATP6V1B2 Antibody [H00000526-D01P] - Analysis of ATP6V1B2 expression in transfected 293T cell line by ATP6V1B2 polyclonal antibody.Lane 1: ATP6V1B2 transfected lysate(56.40 KDa).Lane 2: Non-transfected lysate.

Product Discontinued

ATP6V1B2 Antibody Summary

• Description: Quality control test: Antibody reactive against mammalian transfected lysate.
• Immunogen: ATP6V1B2 (AAH30640.1, 1 a.a. - 511 a.a.) full-length human protein. MALRAMRGIVNGAAPELPVPTGGPAVGAQEQALAVSRNYLSQPRLTYKTVSGVNGPLVILDHVKFPRYAEIVHLTLPDGTKRSGQVLEVSGSKAVVQVFEGTSGIDAKKTSCEFTGDILRTPVSEDMLGRVFNGSGKPIDRGPVVLAEDFLDIMGQPINPQCRIYPEEMIRTGISAIDGMNSIARGQKIPIFSAAGLPHNEIAAQICRQAGLVKKSKDVVDYSEENFAIVFAAMGVNMETARFFKSDFEENGSMDNVCLFLNLANDPTIERIITPRLALTTAEFLAYQCEKHVLVILTDMSSYAEALREVSAAREEVPGRRGFPGYMYTDLATIYERAGRVGGRNGSITQIPILTMPNDDITHPIPDLTGYITEGQIYVDRQLHNRQIYPPINVLPSLSRLMKSAIGEGMTRKDHADVSNQLYACYAIGKDVQAMKAVVGEEALTSDDLLYLEFLQKFERNFIAQGPYENRTVFETLDIGWQLLRIFPKEMLKRIPQSTLSEFYPRDSAKH
• Specificity: ATP6V1B2 - ATPase, H+ transporting, lysosomal 56/58kDa, V1 subunit B2,
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: ATP6V1B2
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Western Blot
• Application Notes: Antibody reactivity against Recombinant Protein with GST tag on ELISA and WB and also on transfected lysate in WB. GST tag alone is used as a negative control.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.4)
• Preservative: No Preservative
• Purity: Protein A purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for ATP6V1B2 Antibody

• 000 subunit
• 56/58kD, isoform 2
• ATP6B2ATPase, H+ transporting, lysosomal (vacuolar proton pump), beta polypeptide
• ATPase, H+ transporting, lysosomal 56/58kDa, V1 subunit B2
• Endomembrane proton pump 58 kDa subunit
• H+ transporting two-sector ATPase
• HO57ATP6B1B2
• vacuolar H+-ATPase 56
• Vacuolar proton pump subunit B 2
• VATB
• V-ATPase B2 subunit
• V-ATPase subunit B 2
• Vma2
• VPP3ATPase, H+ transporting, lysosomal 56/58kDa, V1 subunit B, isoform 2
• V-type proton ATPase subunit B, brain isoform

Background

This gene encodes a component of vacuolar ATPase (V-ATPase), a multisubunit enzyme that mediates acidification of eukaryotic intracellular organelles. V-ATPase dependent organelle acidification is necessary for such intracellular processes as protein sorting, zymogen activation, receptor-mediated endocytosis, and synaptic vesicle proton gradient generation. V-ATPase is composed of a cytosolic V1 domain and a transmembrane V0 domain. The V1 domain consists of three A, three B, and two G subunits, as well as a C, D, E, F, and H subunit. The V1 domain contains the ATP catalytic site. The protein encoded by this gene is one of two V1 domain B subunit isoforms and is the only B isoform highly expressed in osteoclasts. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for ATP6V1B2 Antibody (H00000526-D01P)(1)

Publication using H00000526-D01P

• Sanchez-Mir L, Franco A, Martin-Garcia R et al. Rho2 palmitoylation is required for plasma membrane localization and proper signaling to the fission yeast cell integrity MAPK pathway. Mol Cell Biol. 2014-05-12 [PMID: 24820419]

Bioinformatics

• Gene Symbol: ATP6V1B2
• Entrez:
  • 526(Human)
• Uniprot:
  • AAH30640.1(Human)