Recombinant Human ATP5F1 His Protein

SDS-Page: ATP5F1 Recombinant Protein [NBP2-51532] - 15% SDS Page (3 ug)

SDS-Page: ATP5F1 Recombinant Protein [NBP2-51532] - 15% SDS Page (3 ug)

• Reactivity: Hu
• Applications: PAGE
• Concentration: 0.5 mg/ml

Recombinant Human ATP5F1 His Protein Summary

• Description: A denatured recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 83-256 of Human ATP5F1
  
  

  Source: E.coli

  Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGSLILYALS KEIYVISAET FTALSVLGVM VYGIKKYGPF VADFADKLNE QKLAQLEEAK QASIQHIQNA IDTEKSQQAL VQKRHYLFDV QRNNIAMALE VTYRERLYRV YKEVKNRLDY HISVQNMMRR KEQEHMINWV EKHVVQSIST QQEKETIAKC IADLKLLAKK AQAQPVM

• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Gene: ATP5PB
• Purity: >80%, by SDS-PAGE

Applications/Dilutions

• Dilutions:
  • SDS-Page
• Application Notes: Denatured protein is most likely not the best option for functional studies. It is better suited for Western Blot (WB) or imaging assays.
• Theoretical MW: 22.6 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 20 mM Tris-HCl buffer (pH 8.0), 0.4M UREA, 10% glycerol
• Preservative: No Preservative
• Concentration: 0.5 mg/ml
• Purity: >80%, by SDS-PAGE

Alternate Names for Recombinant Human ATP5F1 His Protein

• ATP synthase B chain, mitochondrial
• ATP synthase subunit b, mitochondrial
• ATP synthase, H+ transporting, mitochondrial F0 complex, subunit b
• ATP synthase, H+ transporting, mitochondrial F0 complex, subunit b, isoform 1
• ATP synthase, H+ transporting, mitochondrial F0 complex, subunit B1
• ATP synthase, H+ transporting, mitochondrial Fo complex, subunit B1
• ATPase subunit b
• cell proliferation-inducing protein 47
• H+-ATP synthase subunit b
• MGC24431
• PIG47

Background

ATP5F1 encodes a subunit of mitochondrial ATP synthase. Mitochondrial ATP synthase catalyzes ATP synthesis, utilizing an electrochemical gradient of protons across the inner membrane during oxidative phosphorylation. ATP synthase is composed of two linked multi-subunit complexes: the soluble catalytic core, F1, and the membrane-spanning component, Fo, comprising the proton channel. The catalytic portion of mitochondrial ATP synthase consists of 5 different subunits (alpha, beta, gamma, delta, and epsilon) assembled with a stoichiometry of 3 alpha, 3 beta, and a single representative of the other 3. The proton channel seems to have nine subunits (a, b, c, d, e, f, g, F6 and 8). This gene encodes the b subunit of the proton channel. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Bioinformatics

• Gene Symbol: ATP5PB