ATP Citrate Lyase Antibody (ST51-07)

Western Blot: ATP Citrate Lyase Antibody (ST51-07) [NBP2-67509] - Analysis of ATP citrate lyase on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: A549 cell lysate Lane 2: CRC cell lysate Lane 3: Hela cell lysate

Immunocytochemistry/Immunofluorescence: ATP Citrate Lyase Antibody (ST51-07) [NBP2-67509] - Staining ATP citrate lyase in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: ATP Citrate Lyase Antibody (ST51-07) [NBP2-67509] - Analysis of mouse thyroid tissue using anti-ATP citrate lyase antibody. Counter stained with hematoxylin.

Flow Cytometry: ATP Citrate Lyase Antibody (ST51-07) [NBP2-67509] - Analysis of Hela cells with ATP citrate lyase antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.

Immunocytochemistry/Immunofluorescence: ATP Citrate Lyase Antibody (ST51-07) [NBP2-67509] - Staining ATP citrate lyase in CRC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunocytochemistry/Immunofluorescence: ATP Citrate Lyase Antibody (ST51-07) [NBP2-67509] - Staining ATP citrate lyase in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: ATP Citrate Lyase Antibody (ST51-07) [NBP2-67509] - Analysis of human breast carcinoma tissue using anti-ATP citrate lyase antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: ATP Citrate Lyase Antibody (ST51-07) [NBP2-67509] - Analysis of human thyroid tissue using anti-ATP citrate lyase citrate lyase antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: ATP Citrate Lyase Antibody (ST51-07) [NBP2-67509] - Analysis of mouse kidney tissue using anti-ATP citrate lyase antibody. Counter stained with hematoxylin.

Product Discontinued

ATP Citrate Lyase Antibody (ST51-07) Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Synthetic peptide within C-terminal human ATP Citrate Lyase. (SwissProt: P53396 Human; SwissProt: Q91V92 Mouse; SwissProt: P16638 Rat)
• Localization: Cytoplasm, nucleoplasm.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: ACLY
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:50-1:200
  • Immunocytochemistry/ Immunofluorescence 1:50-1:200
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Immunoprecipitation
  • Western Blot 1:500-1:2000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: TBS (pH7.4), 0.05% BSA, 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for ATP Citrate Lyase Antibody (ST51-07)

• ACLEC 2.3.3.8
• ACLY
• ATP Citrate Lyase
• ATP citrate synthase
• ATP-citrate (pro-S-)-lyase
• ATP-citrate synthase
• ATPCL
• Citrate cleavage enzyme
• CLATP
• EC 2.3.3.8

Background

ATP citrate lyase (ACLY), also known as ATP citrate synthase, is the primary enzyme responsible for the synthesis of cytosolic acetyl-CoA in many tissues. The enzyme is a tetramer of four identical subunits. It catalyzes the formation of acetyl-CoA and oxaloacetate from citrate and CoA with a concomitant hydrolysis of ATP to ADP and phosphate. One of these products, acetyl-CoA, serves several important biosynthetic pathways, including lipogenesis and cholesterogenesis. In nervous tissue, ATP citrate-lyase may be involved in the biosynthesis of acetylcholine. NDPK has been found to phosphorylate ACLY and insulin to increase phosphorylation of ACLY (2).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ACLY