ATF7 Antibody - BSA Free

Immunoprecipitation: ATF7 Antibody [NBP1-30071] - Whole cell lysate from HeLa cells. ATF7 was also immunoprecipitated by rabbit anti-ATF7 antibody. For blotting immunoprecipitated ATF7, the ReliaBLOT(R) Reagents and Procedures were used.

• Reactivity: Hu, Pm-Or
• Applications: WB, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

ATF7 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit ATF7 Antibody - BSA Free (NBP1-30071) is a polyclonal antibody validated for use in WB and IP. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: maps to a region between residue 444 and 494 of human activating transcription factor 7 using the numbering given in entry CAA40483.1
• Predicted Species: Orangutan (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: ATF7
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation 10-15 ug/mg lysate
  • Western Blot 1:2000-1:10000

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Tris-Citrate/Phosphate (pH 7.0 - 8.0)
• Preservative: 0.09% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for ATF7 Antibody - BSA Free

• activating transcription factor 7ATFATranscription factor ATF-A
• cAMP-dependent transcription factor ATF-7
• cyclic AMP-dependent transcription factor ATF-7
• MGC57182

Background

Eukaryotic gene transcription is regulated by sequence-specific transcription factors, which bind modular cis acting promoter and enhancer elements. The cAMP response element (CRE) consists of the palindromic octanucleotide TGACGTCA. There are several CRE binding proteins within the ATF/CREB family, including CREB-1, CREB-2 (also designated ATF-4), ATF-1, ATF-2 and ATF-3. A novel basic leucine zipper (bZIP) protein, designated ATF-7, is closely related to members of the ATF/CREB family of bZIP proteins, with highest homology to ATF-4. ATF-7 physically interacts with the PRL-1 protein-tyrosine phosphatase (PTPase), which is a predominately nuclear, farnesylated PTPase. ATF-7 homodimers bind specifically to CRE elements. ATF-7 is expressed in a number of different tissues and is expressed in association with differentiation. ATF-7 and PRL-1 interact with each other through the bZIP region of ATF-7 and the phosphatase domain of PRL-1. In addition, PRL-1 is able to dephosphorylate ATF-7 in vitro.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ATF7