ATF5 Antibody

Western Blot: ATF5 Antibody [NBP1-51908] - Staining of Mouse (A) and Rat (B) Skeletal Muscle lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Western Blot: ATF5 Antibody [NBP1-51908] - Analysis of ATF5 antibody in human heart lysate (RIPA buffer, 35 ug total loaded per lane) using NBP1-51908 at 1.5 ug/ml. Primary incubated for 1 hour. ATF5 antibody detected by western blot using chemiluminescence.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ELISA
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

ATF5 Antibody Summary

• Description: Novus Biologicals Goat ATF5 Antibody (NBP1-51908) is a polyclonal antibody validated for use in WB and ELISA. Anti-ATF5 Antibody: Cited in 3 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Peptide with sequence EVYKARSQRTRSC corresponding to C-Terminus according to NP_036200.2.
• Epitope: EVYKARSQRTRSC
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: ATF5
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Peptide ELISA Detection limit 1:32000
  • Western Blot 1 - 3 ug/ml
• Application Notes: WB: Approx. 30 kDa band observed in human heart lysates (calculated MW of 30.7 kDa band according to NP_036200.2).

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for ATF5 Antibody

• activating transcription factor 5FLJ34666
• ATFXHMFN0395
• cAMP-dependent transcription factor ATF-5
• cyclic AMP-dependent transcription factor ATF-5
• Transcription factor ATFx

Background

Transcriptional activator which binds the cAMP response element (CRE) (consensus: 5'-GTGACGT[AC][AG]-3'), a sequence present in many viral and cellular promoters and blocks the differentiation of neuroprogenitor cells into neurons. Its transcriptional activity is enhanced by CCND3 and slightly inhibited by CDK4

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for ATF5 Antibody (NBP1-51908)(3)

Publications using NBP1-51908

• Sampath P, Pritchard DK, Pabon L et al. A hierarchical network controls protein translation during murine embryonic stem cell self-renewal and differentiation. Cell Stem Cell. 2008-05-08 [PMID: 18462695] (WB, Mouse)
  
  Details:
  WB (mouse embryonic stem cells and embryoid bodies): Fig. 5A, B, F.
• Pascual M, Gomez-Lechon MJ, Castell JV, Jover R. ATF5 is a highly abundant liver-enriched transcription factor that cooperates with constitutive androstane receptor in the transactivation of CYP2B6: implications in hepatic stress responses. Drug Metab Dispos. 2008-06-01 [PMID: 18332083] (WB, Human)
  
  Details:
  WB (human AD-ATF5 infected HepG2 cells), Fig. 3B.
• Peters CS, Liang X, Li S et al. ATF-7, a novel bZIP protein, interacts with the PRL-1 protein-tyrosine phosphatase. J Biol Chem 2001-04-27 [PMID: 11278933]

Bioinformatics

• Gene Symbol: ATF5
• Entrez:
  • 22809(Human)
  • 107503(Mouse)
  • 282840(Rat)
• Uniprot:
  • NP_036200.2(Human)