ATF2 Antibody

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Western Blot: ATF2 Antibody [NB100-835] - Analysis of AFT2 in human placenta lysate (RIPA buffer, 35ug total protein per lane) using this antibody at 0.5 ug/ml.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, PEP-ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

ATF2 Antibody Summary

Immunogen
Peptide with sequence C-IVMAPSSQSQPSGS corresponding to C-Terminus according to NP_001871.2.
Localization
Nuclear
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
ATF2
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Western Blot 0.3 - 1 ug/ml
  • Peptide ELISA Detection limit 1:32000
Application Notes
WB: Approx. 55 kDa band and 30 kDa band bands observed in lysates of human placenta. These bands correspond to earlier findings in scientific literature (PMID: 11932306). Approx. 75 kDa band observed in nuclear lysates of HeLa, HepG2 and K562 is reported in scientific literature (PMID: 19331149).
Positive Control
HeLa Lysate (NB800-PC1)
Publications
Read Publications using NB100-835.

Reactivity Notes

Predicted cross-reactivity based on sequence identity: Rat, Canine, Bovine.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
0.5 mg/ml Tris (pH 7.3) and 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for ATF2 Antibody

  • activating transcription factor 2formerly
  • ATF2
  • cAMP responsive element binding protein 2
  • cAMP-responsive element-binding protein 2
  • CREB2
  • CREBP1
  • CRE-BP1
  • cyclic AMP-dependent transcription factor ATF-2
  • HB16
  • MGC111558
  • mXBP
  • TREB7

Background

ATF2 (Activating Transcription Factor 2, CREBP, HB16, CREB2, TREB7) is a member of the ATF/CREB family of basic region leucine zipper DNA binding proteins that regulates transcription by binding to a consensus cAMP response element (CRE) in the promoter of various viral and cellular genes. Many of these genes are important in cell growth and differentiation, and in stress and immune responses. ATF2 is a nuclear protein that binds DNA as a dimer and can form dimers with members of the ATF/CREB and Jun/Fos families. It is a stronger activator as a heterodimer with cJun than as a homodimer. Several isoforms of ATF2 arise by differential splicing. The stable native full length ATF2 is transcriptionally inactive as a result of an inhibitory direct intramolecular interaction of its carboxy terminal DNA binding domain with the amino terminal transactivation domain. Following dimerization ATF2 becomes a short lived protein that undergoes ubiquitination and proteolysis, seemingly in a protein phosphatase-dependent mechanism. Stimulation of the transcriptional activity of ATF2 occurs following cellular stress induced by several genotoxic agents, inflammatory cytokines, and UV irradiation. This activation requires phosphorylation of two threonine residues in ATF2 by both JNK/SAP kinase and p38 MAP kinase. ATF2 is abundantly expressed in brain.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for ATF2 Antibody (NB100-835)(2)

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Positive Control Lysate(s)

Secondary Antibodies

 

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Bioinformatics

Gene Symbol ATF2
Entrez
Uniprot