ATF2 Antibody

Western Blot: ATF2 Antibody [NB100-835] - Human primary astrocyte lysates probed with ATF2 antibody. WB image submitted by a verified customer review.

Western Blot: ATF2 Antibody [NB100-835] - Staining of HepG2 (A), K562 (B) and HeLa (C) nuclear lysates (35 ug protein in RIPA buffer). Antibody at 1 ug/mL. Detected by chemiluminescence.

• Reactivity: Hu, Rt, Bv, Ca
• Applications: WB, ELISA
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

ATF2 Antibody Summary

• Description: Novus Biologicals Goat ATF2 Antibody (NB100-835) is a polyclonal antibody validated for use in WB and ELISA. Anti-ATF2 Antibody: Cited in 2 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Peptide with sequence C-IVMAPSSQSQPSGS corresponding to C-Terminus according to NP_001871.2.
• Localization: Nuclear
• Predicted Species: Rat (100%), Canine (100%), Bovine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: ATF2
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Peptide ELISA Detection limit 1:32000
  • Western Blot 0.3 - 1 ug/mL
• Application Notes: WB: Approx. 55 kDa band and 30 kDa band bands observed in lysates of human placenta. These bands correspond to earlier findings in scientific literature (PMID: 11932306). Approx. 75 kDa band observed in nuclear lysates of HeLa, HepG2 and K562 is reported in scientific literature (PMID: 19331149).
• Control:

  HeLa Whole Cell Lysate

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for ATF2 Antibody

• activating transcription factor 2formerly
• ATF2
• cAMP responsive element binding protein 2
• cAMP-responsive element-binding protein 2
• CREB2
• CREBP1
• CRE-BP1
• cyclic AMP-dependent transcription factor ATF-2
• HB16
• MGC111558
• mXBP
• TREB7

Background

ATF2 (Activating Transcription Factor 2, CREBP, HB16, CREB2, TREB7) is a member of the ATF/CREB family of basic region leucine zipper DNA binding proteins that regulates transcription by binding to a consensus cAMP response element (CRE) in the promoter of various viral and cellular genes. Many of these genes are important in cell growth and differentiation, and in stress and immune responses. ATF2 is a nuclear protein that binds DNA as a dimer and can form dimers with members of the ATF/CREB and Jun/Fos families. It is a stronger activator as a heterodimer with cJun than as a homodimer. Several isoforms of ATF2 arise by differential splicing. The stable native full length ATF2 is transcriptionally inactive as a result of an inhibitory direct intramolecular interaction of its carboxy terminal DNA binding domain with the amino terminal transactivation domain. Following dimerization ATF2 becomes a short lived protein that undergoes ubiquitination and proteolysis, seemingly in a protein phosphatase-dependent mechanism. Stimulation of the transcriptional activity of ATF2 occurs following cellular stress induced by several genotoxic agents, inflammatory cytokines, and UV irradiation. This activation requires phosphorylation of two threonine residues in ATF2 by both JNK/SAP kinase and p38 MAP kinase. ATF2 is abundantly expressed in brain.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for ATF2 Antibody (NB100-835)(2)

Publications using NB100-835

• Bailey J, Phillips RJ, Pollard AJ et al. Characterization and functional analysis of cAMP response element modulator protein and activating transcription factor 2 (ATF2) isoforms in the human myometrium during pregnancy and labor: identification of a novel ATF2 species with potent transactivation properties. J Clin Endocrinol Metab 2002-04-01 [PMID: 11932306]
• Monzen K, Hiroi Y, Kudoh S et al. Smads, TAK1, and their common target ATF-2 play a critical role in cardiomyocyte differentiation. J Cell Biol 2001-05-14 [PMID: 11352931]

Review for ATF2 Antibody (NB100-835) (1)

Average Rating: 4

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Pauline Jeannot WB Human 11/11/2021

Summary

• Application: Western Blot
• Sample Tested: primary astrocytes
• Species: Human
• Lot: P1

Bioinformatics

• Gene Symbol: ATF2
• Entrez:
  • 1386(Human)
  • 81647(Rat)
• Uniprot:
  • NP_001871.(Human)