ATF2 [p Thr71] Antibody (103C411.2)

Western Blot: ATF2 [p Thr71] Antibody (103C411.2) [NB100-56594] - Detection of ATF2 phosphorylation in HeLa cell lysate with phospho ATF2 antibody. Lane A. Untreated cell lysate; Lane B. HeLa cells treated with TNF for 1 hr.

• Reactivity: Hu
• Applications: WB, Flow
• Clone: 103C411.2
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

ATF2 [p Thr71] Antibody (103C411.2) Summary

• Immunogen: This antibody was generated by immunizing mice with a synthetic peptide containing phosphorylated threonine at position 71 of human ATF-2.
• Modification: p Thr71
• Specificity: This monoclonal antibody is specific for phospho (Thr71) ATF2. It cannot be used to test for non-phosphorylated ATF2.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: ATF2
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:10-1:1000
  • Western Blot 2 ug/ml

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS containing 0.05% BSA
• Preservative: 0.05% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Protein G purified

Alternate Names for ATF2 [p Thr71] Antibody (103C411.2)

• activating transcription factor 2formerly
• ATF2
• cAMP responsive element binding protein 2
• cAMP-responsive element-binding protein 2
• CREB2
• CREBP1
• CRE-BP1
• cyclic AMP-dependent transcription factor ATF-2
• HB16
• MGC111558
• mXBP
• TREB7

Background

ATF-2 is a member of the group of bZip transcription factors. Heterodimer formation between members of the bZip group is common and is believed to add diversity to the cis-acting elements at which binding of the dimers is directed. Specifically, ATF-2 may dimerize with c-Jun, as occurs in response to E1a, and in so doing shift the binding preference of c-Jun toward ATF/CRE sites. Deletion analysis has indicated that the N-terminal region of ATF-2 containing threonine at residues 69 and 71 are essential for this purpose. These threonine residues are phosphorylated by JNK/SAPK for transcriptional activation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Review for ATF2 Antibody (NB100-56594) (1)

Average Rating: 5

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Verified Customer WB Human 08/09/2021

Summary

• Application: Western Blot
• Sample Tested: HEK293T Cells (Human Kidney)
• Species: Human
• Lot: 103C411.2

Bioinformatics

• Gene Symbol: ATF2
• Entrez:
  • 1386(Human)
• Uniprot:
  • P15336()