Ataxin-10 Recombinant Protein Antigen

• Reactivity: Hu
• Applications: AC

Ataxin-10 Recombinant Protein Antigen Summary

• Description: A recombinant protein antigen with a N-terminal His6-ABP tag corresponding to human ATXN10.
  
  

  Source: E. coli

  Amino Acid Sequence: KHPESEWPFLIITDLFLKSPELVQAMFPKLNNQERVTLLDLMIAKITSDEPLTKDDIPVFLRHAELIASTF

  Fusion Tag: N-terminal His6ABP (ABP = Albumin Binding Protein derived from Streptococcal Protein G)

  This product is intended to be used as a blocking antigen for antibody competition assays. Any other use of this antigen is done at the risk of the user. The use of this product for commercial production is strictly prohibited. Please contact technical support if you have any questions.

• Source: E. coli
• Protein/Peptide Type: Recombinant Protein Antigen
• Gene: ATXN10
• Purity: >80% by SDS-PAGE and Coomassie blue staining

Applications/Dilutions

• Dilutions:
  • Antibody Competition 10 - 100 molar excess
• Application Notes: This recombinant antigen is only intended to be used as a blocking agent to confirm antibody specificity with the corresponding antibody, catalog number NBP2-14336.

  It is purified by IMAC chromatography, and the expected concentration is greater than 0.5 mg/ml.

  For current lot information, including availability, please contact our technical support team click nb-technical@bio-techne.com

• Theoretical MW: 26 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS and 1M Urea, pH 7.4.
• Preservative: No Preservative
• Purity: >80% by SDS-PAGE and Coomassie blue staining

Alternate Names for Ataxin-10 Recombinant Protein Antigen

• ataxin 10
• ataxin-10
• Brain protein E46 homolog
• E46L
• FLJ37990
• HUMEEP
• SCA10spinocerebellar ataxia 10
• Spinocerebellar ataxia type 10 protein

Background

Pentanucleotide repeat expansions in the ATXN10/SCA10 gene have been linked to spinocerebellar ataxia type 10, a disorder that is characterized by gait ataxia, cognitive development, and seizures. ATXN10/SCA10 is an evolutionarily conserved protein whose function has not been identified. It is proposed that the repeats in ATXN10/SCA10 may form unusual RNA hairpin structures that result in toxic RNA that may have biological effects such as sequestering of proteins, activation of enzymes, and deregulation of gene expression via the RNA interference pathway. ATXN10/SCA10 is also known as ataxin-10, spinocerebellar ataxia type 10 protein, brain protein E46 homolog, E46L, and FLJ37990.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Bioinformatics

• Gene Symbol: ATXN10