Astrin Antibody

Western Blot: Astrin Antibody [NBP1-85261] - Analysis in control (vector only transfected HEK293T lysate) and SPAG5 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).

Immunohistochemistry-Paraffin: Astrin Antibody [NBP1-85261] - Staining of human testis shows moderate cytoplasmic positivity in cells in seminiferous ducts.

Western Blot: Astrin Antibody [NBP1-85261] - Analysis in human cell line A-549.

Immunohistochemistry-Paraffin: Astrin Antibody [NBP1-85261] - Staining of human testis shows moderate cytoplasmic positivity in cells in seminiferous ducts.

Product Discontinued

Astrin Antibody Summary

• Immunogen: This antibody was developed against Recombinant Protein corresponding to amino acids: KSGELISLREEVTHLTRSLRRAETETKVLQEALAGQLDSNCQPMATNWIQEKVWLSQEVDKLRVMFLEMKNEKEKLMIKFQSHRNILEENLRRSDKELEKLDDIVQHIYKTLLSIPEVVRGCKELQGLLEF
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: SPAG5
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:50 - 1:200
  • Immunohistochemistry-Paraffin 1:20-1:50
  • Western Blot 0.04 - 0.4 ug/ml
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (80%)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for Astrin Antibody

• Astrin
• DEEPEST
• hMAP126
• MAP126mitotic spindle coiled-coil related protein
• mitotic spindle associated protein p126
• Mitotic spindle-associated protein p126
• sperm associated antigen 5
• sperm-associated antigen 5

Background

Astrin encodes a protein associated with the mitotic spindle apparatus. The encoded protein may be involved in the functional and dynamic regulation of mitotic spindles.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: SPAG5