ASK1 Antibody - BSA Free

Western Blot: ASK1 Antibody [NB100-81788] - Analysis of extracts from 293 and Hela cells using NB100-81788

Immunocytochemistry/ Immunofluorescence: ASK1 Antibody - BSA Free [NB100-81788] - Immunofluorescence staining of methanol-fixed Hela cells using NB100-81788.

Immunohistochemistry-Paraffin: ASK1 Antibody [NB100-81788] - Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue, using NB100-81788(left) or the same antibody preincubated with blocking peptide(right).

• Reactivity: Hu, Mu, Rt
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

ASK1 Antibody - BSA Free Summary

• Immunogen: The antiserum was produced against synthesized non-phosphopeptide derived from human ASK1 around amino acids 964~968 (S-I-S-L-P).
• Specificity: Detects endogenous levels of total ASK1 protein.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: MAP3K5
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:200
  • Immunohistochemistry 1:50-1:100
  • Immunohistochemistry-Paraffin 1:50-1:100
  • Western Blot 1:500-1:1000
• Theoretical MW: 155 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control:

  ASK1 Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol
• Preservative: 0.02% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for ASK1 Antibody - BSA Free

• Apoptosis signal-regulating kinase 1
• ASK1
• ASK-1
• ASK1MEKK 5
• EC 2.7.11
• MAP/ERK kinase kinase 5
• MAPK/ERK kinase kinase 5
• MAPKKK5EC 2.7.11.25
• MEK kinase 5
• MEKK5apoptosis signal regulating kinase 1
• mitogen-activated protein kinase kinase kinase 5

Background

Mitogen activated protein (MAP) kinase cascades are activated in response to various extracellular stimuli including cytokines, growth factors and environmental stresses. A novel MAP kinase kinase kinase (MAPKKK) was recently identified and designated ASK1 (for apoptosis signal-regulating kinase 1). ASK1 activates two different subgroups of MAPKK, MKK4 and MKK6, which in turn activate c-Jun N terminal kinase (JNK) and p38 MAP kinase, respectively. ASK1 is activated by TNFR and Fas through the interaction with members of the TRAF family and Fas associated protein Daxx. Over expression of ASK1 induces apoptotic cell death, and a catalytically inactive form of ASK1 inhibits TNF alpha-induced apoptosis. ASK1 is expressed in variety of human and mouse tissues.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for ASK1 Antibody (NB100-81788)(1)

Publication using NB100-81788

• Zhiyong Xu, Chunyi Guo, Qiaoli Ye, Yueli Shi, Yihui Sun, Jie Zhang, Jiaqi Huang, Yizhou Huang, Chunlai Zeng, Xue Zhang, Yuehai Ke, Hongqiang Cheng Endothelial deletion of SHP2 suppresses tumor angiogenesis and promotes vascular normalization Nature Communications 2021-11-02 [PMID: 34728626]

Bioinformatics

• Gene Symbol: MAP3K5
• Entrez:
  • 4217(Human)
  • 26408(Mouse)
• Uniprot:
  • Q99683()