Home » ASK1 » ASK1 Research Products » ASK1 Antibody

ASK1 Antibody

 Submit a Review

Images

 
(
Enlarge)
Western Blot: ASK1 Antibody [NB100-81788] - Analysis of extracts from 293 and Hela cells using ASK1(Ab-966) antibody
Western Blot: ASK1 Antibody [NB100-81788] - Analysis of extracts from 293 and Hela cells using ASK1(Ab-966) antibody
Immunocytochemistry/ Immunofluorescence: ASK1 Antibody [NB100-81788] - Staining of methanol-fixed Hela cells using ASK1(Ab-966) antibody
Immunohistochemistry-Paraffin: ASK1 Antibody [NB100-81788] - Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue, using ASK1 (Ab-966) antibody.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Order Details

100% Guarantee on every product

ASK1 Antibody Summary

Immunogen
The antiserum was produced against synthesized non-phosphopeptide derived from human ASK1 around aa. 966 (S-I-S-L-P).
Specificity
ASK1 (specific to a.a. 966) detects endogenous levels of total ASK1 protein.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
MAP3K5
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:1000
  • Immunocytochemistry/Immunofluorescence 1:100-1:200
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50-1:100
Theoretical MW
155 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control
ASK1 Overexpression Lysate

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, and 50% glycerol.
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for ASK1 Antibody

  • Apoptosis signal-regulating kinase 1
  • ASK1
  • ASK-1
  • ASK1MEKK 5
  • EC 2.7.11
  • MAP/ERK kinase kinase 5
  • MAP3K5
  • MAPK/ERK kinase kinase 5
  • MAPKKK5EC 2.7.11.25
  • MEK kinase 5
  • MEKK5
  • MEKK5apoptosis signal regulating kinase 1
  • mitogen-activated protein kinase kinase kinase 5

Background

Mitogen activated protein (MAP) kinase cascades are activated in response to various extracellular stimuli including cytokines, growth factors and environmental stresses. A novel MAP kinase kinase kinase (MAPKKK) was recently identified and designated ASK1 (for apoptosis signal-regulating kinase 1). ASK1 activates two different subgroups of MAPKK, MKK4 and MKK6, which in turn activate c-Jun N terminal kinase (JNK) and p38 MAP kinase, respectively. ASK1 is activated by TNFR and Fas through the interaction with members of the TRAF family and Fas associated protein Daxx. Over expression of ASK1 induces apoptotic cell death, and a catalytically inactive form of ASK1 inhibits TNF alpha-induced apoptosis. ASK1 is expressed in variety of human and mouse tissues.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

JNK1 Antibody
(228601) [Unconjugate
MAB17761
JNK1 Antibody (228601) [Unconjugated]
(2 Reviews)
(3 Publications)
Species: Hu, Mu, Rt
Applications: WB, ICC
TNF-alpha
[Unconjugated]
210-TA
TNF-alpha [Unconjugated]
(35 Reviews)
(651 Publications)
Species: Hu
ERK2 Antibody
(OTI6E5)
NBP1-47842
Western Blot: ERK2 Antibody (6E5) [NBP1-47842] - Analysis of extracts (35ug) from 9 different cell lines by using anti-ERK2 monoclonal antibody.
Immunohistochemistry-Paraffin: ERK2 Antibody (6E5) [NBP1-47842] - Endometrium tissue within the normal limits using antiMAPK1mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min,Dilution 1:50)
Western Blot: ERK2 Antibody (6E5) [NBP1-47842] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ERK2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ERK2.
Species: Hu, Mu, Rt, Ca, Pm
Applications: WB, IHC, IHC-P, IP
p38 alpha [p
Thr180, p Tyr182] Ant
NB500-138
Western Blot: p38 [p Thr180, p Tyr182] Antibody [NB500-138] - Hela cell lysates that had been treated with UV or untreated (Control) showing specific immunolabeling of the -39 kDa p38 MAPK protein phosphorylated at Thr180/Tyr182.
Immunohistochemistry-Paraffin: p38 [p Thr180, p Tyr182] Antibody [NB500-138] - Immunostaining of human breast cancer tissue showing p38 when phosphorylated at Thr180/Tyr182 in red.
Western Blot: p38 [p Thr180, p Tyr182] Antibody [NB500-138] - Western blot of HeLa cell lysates that had been treated with UV or untreated (Control) showing specific immunolabeling of the ~39k p38 MAPK protein phosphorylated at Thr180/Tyr182.
(12 Publications)
Species: Hu, Mu, Rt, Ze
Applications: WB, IHC, IHC-P, Flow-IC
Thioredoxin-1
Antibody
NBP2-52983
Immunocytochemistry/Immunofluorescence: Thioredoxin Antibody [NBP2-52983] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. The cells were incubated with anti-Thioredoxin at a 1:100 dilution overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Alpha tubulin (DM1A) [NB100-690] was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse Dylight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.
Immunocytochemistry/Immunofluorescence: Thioredoxin Antibody [NBP2-52983] - NIH-3T3 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton-X100. The cells were incubated with anti-Thioredoxin at 5 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.
Immunocytochemistry/Immunofluorescence: Thioredoxin Antibody [NBP2-52983] - PC12 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton-X100. The cells were incubated with anti-Thioredoxin at 5 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.
Species: Hu, Mu
Applications: WB, Flow, ICC/IF, IHC, IHC-P
POLDIP2 Antibody
NBP1-68874
Western Blot: POLDIP2 Antibody [NBP1-68874] - Staining of K562 (A), MCF7 (B) and Mouse Liver (C) lysates (35ug protein in RIPA buffer). Primary incubation was overnight at 4C. Detected by chemiluminescence.
Western Blot: POLDIP2 Antibody [NBP1-68874] - (1ug/ml) Analysis of MCF7 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
(1 Publication)
Species: Hu, Mu, Rt
Applications: WB, PEP-ELISA
Crk Antibody
(3G11E8)
NBP2-37568
Western Blot: Crk Antibody (3G11E8) [NBP2-37568] - Western blot analysis using CRK mAb against HEK293 (1) and CRK(AA: 1-204)-hIgGFc transfected HEK293 (2) cell lysate.
Immunocytochemistry/Immunofluorescence: Crk Antibody (3G11E8) [NBP2-37568] - Immunofluorescence analysis of 3T3-L1 cells using CRK mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Immunohistochemistry: Crk Antibody (3G11E8) [NBP2-37568] - Immunohistochemical analysis of paraffin-embedded lung cancer tissues using CRK mouse mAb with DAB staining.
Species: Hu
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-P
GRAP2 Antibody
(OTI1G2)
NBP2-00764
Western Blot: GRAP2 Antibody (1G2) [NBP2-00764] Analysis of extracts (35ug) from 9 different cell lines by using anti-GRAP2 monoclonal antibody.
Immunohistochemistry-Paraffin: GRAP2 Antibody (1G2) [NBP2-00764] - Staining of paraffin-embedded Human endometrium tissue using anti-GRAP2 mouse monoclonal antibody.
Flow Cytometry: GRAP2 Antibody (1G2) [NBP2-00764] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-GRAP2 antibody, and then analyzed by flow cytometry.
Species: Hu, Mu, Rt
Applications: WB, Flow, IHC, IHC-P
AHSA1 Antibody
(1A2-A8)
H00010598-M01
Western Blot: AHSA1 Antibody (1A2-A8) [H00010598-M01] - AHSA1 monoclonal antibody (M01), clone 1A2-A8 Analysis of AHSA1 expression in HepG2.
Sandwich ELISA: AHSA1 Antibody (1A2-A8) [H00010598-M01] - Detection limit for recombinant GST tagged AHSA1 is 3 ng/ml as a capture antibody.
(6 Publications)
Species: Hu, Mu, Rt
Applications: WB, ELISA, ICC/IF, S-ELISA
AIMP2 Antibody
NBP1-81575
Western Blot: AIMP2 Antibody [NBP1-81575] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp. Lane 4: Human plasma (IgG/HSA depleted). Lane 5: Human liver tissue. Lane 6: Human tonsil tissue
Immunocytochemistry/Immunofluorescence: AIMP2 Antibody [NBP1-81575] - Staining of human cell line A549 shows localization to cytosol. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: AIMP2 Antibody [NBP1-81575] - Staining of human testis shows weak to moderate cytoplasmic positivity in cells in seminiferous ducts.
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
c-jun Antibody
(5B1)
NBP2-37576
Western Blot: c-jun Antibody (5B1) [NBP2-37576] - Analysis using c-Jun mouse mAb against NIH/3T3 (1) and Cos7 (2) cell lysate.
Immunocytochemistry/Immunofluorescence: c-jun Antibody (5B1) [NBP2-37576] - Analysis of PC-2 cells using c-Jun mouse mAb (green). Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Immunohistochemistry-Paraffin: c-jun Antibody (5B1) [NBP2-37576] - Analysis of human rectum cancer tissues using c-Jun mouse mAb with DAB staining.
Species: Hu, Mu, Pm
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-P
MKK4/MEK4 Antibody
(OTI8A8)
NBP1-47839
Western Blot: MKK4/MEK4 Antibody (OTI8A8) [NBP1-47839] - Analysis of extracts (35ug) from 9 different cell lines by using anti-MEK4 monoclonal antibody.
Immunocytochemistry/Immunofluorescence: MKK4/MEK4 Antibody (OTI8A8) [NBP1-47839] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY MEK4.
Immunohistochemistry-Paraffin: MKK4/MEK4 Antibody (OTI8A8) [NBP1-47839] - Staining of paraffin-embedded Human thyroid tissue using anti-MEK4 mouse monoclonal antibody.
Species: Hu, Mu, Rt, Ca
Applications: WB, Flow, ICC/IF, IHC, IHC-P, IP
SGSM3 Antibody
NBP2-13304
Western Blot: SGSM3 Antibody [NBP2-13304] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Human cell line RT-4
Immunocytochemistry/Immunofluorescence: SGSM3 Antibody [NBP2-13304] - Immunofluorescent staining of human cell line MCF7 shows localization to the Golgi apparatus.
Immunohistochemistry-Paraffin: SGSM3 Antibody [NBP2-13304] - Staining of human stomach, lower shows strong granular cytoplasmic positivity in glandular cells.
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
TRAF-2 Antibody
NB100-56173
Western Blot: TRAF-2 Antibody [NB100-56173] - Analysis of TRAF-2 in normal human tissues using this antibody. TRAF-2 is observed at ~60-64 kDa. Additional bands of lower molecular weight were seen in some cases, and may represent TRAF-2 degradation fragments.
Immunocytochemistry/Immunofluorescence: TRAF-2 Antibody [NB100-56173] - Immunostaining of HN30 and MCF-12a cells for TRAF2 3 days post-transfectionMCF-12a NT (DAPI): non-treated MCF-12a DAPI stained, MCF-12a NT (TRAF2-FITC): non-treated MCF-12a FITC stained for TRAF2, MCF-12a pNSP 5a3a (DAPI): MCF-12a transfected with pcDNA 3.0 NSP 5a3a DAPI stained, MCF-12a pNSP 5a3a (TRAF2-FITC): MCF-12a transfected with pcDNA 3.0 NSP 5a3a FITC stained for Traf-2, HN30 NT (DAPI): non-treated HN30 DAPI stained, HN30 NT (TRAF2-FITC): non-treated HN30 FITC stained for TRAF2, HN30 pNSP 5a3a (DAPI): HN30 transfected with pcDNA 3.0 NSP 5a3a DAPI stained, HN30 pNSP 5a3a (TRAF2-FITC): HN30 transfected with pcDNA 3.0 NSP 5a3a FITC stained for TRAF2. White arrows indicate apoptotic bodies. Image collected and cropped by Cite Ab from the following publication (A novel dual signaling axis for NSP 5a3a induced apoptosis in head and neck carcinoma. Oncotarget (2011) licensed under a CC-BY licence.
Immunohistochemistry-Paraffin: TRAF-2 Antibody [NB100-56173] - Formalin-fixed, paraffin-embedded human colon carcinoma tissue sections stained for TRAF2 using this antibody at 1:2000. Hematoxylin-eosin counterstain.
(3 Publications)
Species: Hu
Applications: WB, Flow, ICC/IF, IHC, IHC-P, IP
MKK3/MEK3 Antibody
(2E12D11)
NBP2-52508
Western Blot: MKK3/MEK3 Antibody (2E12D11) [NBP2-52508] - Analysis using MAP2K3 mAb against human MAP2K3 (AA: 1-138) recombinant protein. (Expected MW is 42.1 kDa)
Immunohistochemistry: MKK3/MEK3 Antibody (2E12D11) [NBP2-52508] - Analysis of paraffin-embedded rectum cancer tissues using MAP2K3 mouse mAb with DAB staining.
Flow Cytometry: MKK3/MEK3 Antibody (2E12D11) [NBP2-52508] - Analysis of Hela cells using MAP2K3 mouse mAb (green) and negative control (red).
Species: Hu
Applications: WB, ELISA, Flow, IHC, IHC-P, CyTOF-ready, ICC, IF
VAC14 Antibody
H00055697-B01P
Western Blot: VAC14 Antibody [H00055697-B01P] - Analysis of VAC14 expression in human liver.
Immunocytochemistry/Immunofluorescence: VAC14 Antibody [H00055697-B01P] - Analysis of purified antibody to VAC14 on HeLa cell. (antibody concentration 10 ug/ml)
Western Blot: VAC14 Antibody [H00055697-B01P] - Analysis of VAC14 expression in transfected 293T cell line by VAC14 polyclonal antibody. Lane 1: VAC14 transfected lysate(86.02 KDa). Lane 2: Non-transfected lysate.
Species: Hu
Applications: WB, ICC/IF
Caspase-3 Antibody
(31A1067) - (Pro and ...
NB100-56708
Western Blot: Caspase-3 Antibody (31A1067) - (Pro and Active) [NB100-56708] - Image of Caspase-3 Antibody (31A1067) - (Pro and Active). Whole cell protein from Jurkat cells treated with and without 2 uM staurosporine as indicated was separated on a 4-15% gel by SDS-PAGE, transferred to 0.2 um PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 5 ug/ml anti-Caspase 3 in 1% milk, and detected with an anti-mouse HRP secondary antibody using a Femto sensitivity chemiluminescence reagent. Note the detection of both pro-caspase 3 at 35 kDa and the cleaved active caspase 3 at 15-17 kDa.
Immunohistochemistry-Paraffin: Caspase-3 Antibody (31A1067) - (Pro and Active) [NB100-56708] - Tissue section of human spleen using 1:200 dilution of Caspase-3 antibody (clone 31A1067). The staining was developed with HRP labeled anti-mouse IgG secondary antibody and DAB reagent, and nuclei of cells were counter-stained with hematoxylin. This Caspase 3 antibody generated primarily a specific cytoplasmic staining in a subset of spleenocytes with some nuclear signal in a few cells.
Simple Western: Caspase-3 Antibody (31A1067) - (Pro and Active) [NB100-56708] - Simple Western lane view shows a specific band for Caspase-3 Antibody (31A1067) - (Pro and Active) in 0.1 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230kDa separation system.
(1 Review)
(155 Publications)
Species: Hu, Mu, Rt, Po, Ch, ChHa, Ma
Applications: WB, Simple Western, EM, Flow, HEStain, IB, ICC/IF, IHC, IHC-Fr, IHC-P, CyTOF-ready, IHC-FrFl, KD, KO
AKT1 [p Ser473]
Antibody (104A282)
NB100-56749
Western Blot: AKT1 [p Ser473] Antibody (104A282) [NB100-56749] - Total protein from mouse 3T3 cells treated with and without PDGF (50 ng/mL) for the indicated times was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/mL anti-AKT1 (NBP2-01725) and 2 ug/mL pS473 AKT1 in 1% BSA in TBST and detected with an anti-mouse HRP secondary antibody using chemiluminescence. Note the detection of phosphorylated AKT1 in response to PDGF treatment compared to total AKT1 protein.
Western Blot: AKT1 [p Ser473] Antibody (104A282) [NB100-56749] - WB of phospho AKT using phospho AKT antibody at 2 ug/mL against untreated (lane 1) and PDGF treated (lane 2) NIH-3T3 lysate. HRP conjugated secondary antibody and ECL substrate solution were used for this test. Image using the Azide and BSA Free form of this antibody.
Immunohistochemistry-Paraffin: AKT1 [p Ser473] Antibody (104A282) [NB100-56749] - IHC analysis of an FFPE human breast carcinoma tissue section using 1:250 dilution of phospho Ser473 AKT1 antibody (clone 104A282) on a Bond Rx autostainer (Leica Biosystems). The assay involved 20 minutes of heat induced antigen retrieval (HIER) with 10 mM sodium citrate buffer (pH 6.0) and endogenous peroxidase quenching using peroxide block. The sections were incubated with primary antibody for 30 minutes. Bond Polymer Refine Detection (Leica Biosystems) and DAB were used for signal detection which followed counterstaining with hematoxylin. Whole slide scanning and capturing of representative images (20X) were performed using Aperio AT2 (Leica Biosystems). This antibody generated a diffused cytoplasmic staining of phosphor-AKT (Ser-473) in the cancer cells as well as the stromal cells. Staining was performed by Histowiz.
(1 Review)
(12 Publications)
Species: Hu, Mu, Rt, Rb
Applications: WB, IHC, IHC-P

Publications for ASK1 Antibody (NB100-81788) (0)

There are no publications for ASK1 Antibody (NB100-81788).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for ASK1 Antibody (NB100-81788) (0)

There are no reviews for ASK1 Antibody (NB100-81788). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for ASK1 Antibody (NB100-81788) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Control Lysate(s)

ASK1 Overexpression Lysate

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Learn about Custom Antibody Labeling

Additional ASK1 Products

Bioinformatics Tool for ASK1 Antibody (NB100-81788)

Discover related pathways, diseases and genes to ASK1 Antibody (NB100-81788). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for ASK1 Antibody (NB100-81788)

Discover more about diseases related to ASK1 Antibody (NB100-81788).
 

Pathways for ASK1 Antibody (NB100-81788)

View related products by pathway.

PTMs for ASK1 Antibody (NB100-81788)

Learn more about PTMs related to ASK1 Antibody (NB100-81788).

Blogs on ASK1.

Check out the latest blog posts on ASK1.
Liver ASK1 activates autophagy to protect against hepatic fat accumulation, non-alcoholic steatohepatitis and fibrosis
By Jamshed Arslan, Pharm. D., PhD. The most common chronic liver disorder worldwide is non-alcoholic fatty liver disease (NAFLD). This obesity-linked disorder can manifest as hepatic fat accumulation (steatosis) wit...  Read full blog post.
IRE1 alpha dependent apoptotic-signaling pathway
Despite in depth characterization of the role of IRE1 alpha (inositol-requiring enzyme 1 alpha) in activating the unfolded protein response (UPR) in the ER - little is known about the molecular mechanisms by which this ER protein has shown to reg...  Read full blog post.

Customers Who Bought This Also Bought

Contact Information

Product PDFs

  SDS
  PDF Datasheet
  Request CofA

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our ASK1 Antibody and receive a gift card or discount.


Bioinformatics

Gene Symbol MAP3K5
Entrez
Uniprot