Asialoglycoprotein Receptor 2 Antibody (OTI2A12) - Azide and BSA Free

Western Blot: Asialoglycoprotein Receptor 2 Antibody (OTI2A12) - Azide and BSA Free [NBP2-70212] - Human ASGR2 over-expressing cells. Western blot image submitted by a verified customer review.

Immunohistochemistry: Asialoglycoprotein Receptor 2 Antibody (OTI2A12) - Azide and BSA Free [NBP2-70212] - Analysis of Carcinoma of Human thyroid tissue. Heat-induced epitope retrieval by 10 mM citric buffer, pH 6.0, 120C for 3 minutes.

Western Blot: Asialoglycoprotein Receptor 2 Antibody (OTI2A12) - Azide and BSA Free [NBP2-70212] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Asialoglycoprotein receptor 2.

Immunohistochemistry: Asialoglycoprotein Receptor 2 Antibody (OTI2A12) - Azide and BSA Free [NBP2-70212] - Analysis of Adenocarcinoma of Human colon tissue. Heat-induced epitope retrieval by 10 mM citric buffer, pH 6.0, 120C for 3 minutes.

Immunohistochemistry: Asialoglycoprotein Receptor 2 Antibody (OTI2A12) - Azide and BSA Free [NBP2-70212] - Analysis of Adenocarcinoma of Human endometrium tissue. Heat-induced epitope retrieval by 10 mM citric buffer, pH 6.0, 120C for 3 minutes.

Immunohistochemistry: Asialoglycoprotein Receptor 2 Antibody (OTI2A12) - Azide and BSA Free [NBP2-70212] - Analysis of Adenocarcinoma of Human ovary tissue. Heat-induced epitope retrieval by 10 mM citric buffer, pH 6.0, 120C for 3 minutes.

Immunohistochemistry: Asialoglycoprotein Receptor 2 Antibody (OTI2A12) - Azide and BSA Free [NBP2-70212] - Analysis of Carcinoma of Human liver tissue. Heat-induced epitope retrieval by 10 mM citric buffer, pH 6.0, 120C for 3 minutes.

Immunohistochemistry: Asialoglycoprotein Receptor 2 Antibody (OTI2A12) - Azide and BSA Free [NBP2-70212] - Analysis of Human lymph node tissue. Heat-induced epitope retrieval by 10 mM citric buffer, pH 6.0, 120C for 3 minutes.

• Reactivity: Hu
• Applications: WB, IHC
• Clone: OTI2A12
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: LYOPH

Asialoglycoprotein Receptor 2 Antibody (OTI2A12) - Azide and BSA Free Summary

• Description: Novus Biologicals Mouse Asialoglycoprotein Receptor 2 Antibody (OTI2A12) - Azide and BSA Free (NBP2-45505) is a monoclonal antibody validated for use in IHC and WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Full length human recombinant protein of human ASGR2 (NP_001172) produced in HEK293T cell.
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: ASGR2
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:150
  • Immunohistochemistry-Paraffin 1:150
  • Western Blot 1:4000
• Theoretical MW: 35 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from PBS (pH 7.3) with 8% Trehalose
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Immunogen affinity purified
• Reconstitution Instructions: we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Alternate Names for Asialoglycoprotein Receptor 2 Antibody (OTI2A12) - Azide and BSA Free

• ASGPR 2
• ASGP-R 2
• ASGPR2
• ASGP-R2
• ASGR2
• asialoglycoprotein receptor 2
• CLEC4H2
• CLEC4H2FLJ60040
• C-type lectin domain family 4 member H2
• HBxAg-binding protein
• HBXBP
• Hepatic lectin H2
• HL-2

Background

Asialoglycoprotein Receptor 2 is a transmembrane protein that plays a critical role in serum glycoprotein homeostasis by mediating the endocytosis and lysosomal degradation of plasma glycoproteins in which the terminal sialic acid residue on their complex carbohydrate moieties has been removed. It is expressed exclusively in hepatic parenchymal cells and calcium is required for ligand binding. Asialoglycoprotein Receptor 2 may facilitate hepatic infection by multiple viruses including hepatitis B, and is also a target for liver-specific drug delivery. Asialoglycoprotein Receptor 2 is involved in hepatitis B, hepatitis C, liver cirrhosis, hepatocellular carcinoma, nephropathy, leukemia, urethritis, autoimmune hepatitis, alcoholic liver cirrhosis, and jaundice.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ASGR2