ASH1 Antibody

Product Discontinued

ASH1 Antibody Summary

• Immunogen: In vivo generated recombinant protein fragment
• Specificity: Drosophila ASH1.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: ASH1L
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Chromatin Immunoprecipitation (ChIP)
  • ELISA 1:100-1:2000
  • Western Blot 1:5000-1:20000
• Application Notes: This product is useful in ELISA and Western Blot. Use in ChIP reported in scientific literature (PMID:29555581).

Reactivity Notes

Drosophila

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 20mM Potassium Phosphate (pH 7.0) and 0.15M NaCl
• Preservative: No Preservative
• Purity: Immunogen affinity purified

Alternate Names for ASH1 Antibody

• Absent small and homeotic disks protein 1 homolog
• ash1 (absent, small, or homeotic)-like (Drosophila)
• ASH1
• ASH1L1
• ASH1-like protein
• EC 2.1.1.43
• FLJ10504
• huASH1
• KMT2HKIAA1420
• Lysine N-methyltransferase 2H
• probable histone-lysine N-methyltransferase ASH1L

Background

Trithorax group (TrxG) protein that has histone methyltransferase activity. Specifically trimethylates 'Lys-4' of histone H3, a specific tag for epigenetic transcriptional activation. May also trimethylate H3 'Lys-9' and H4 'Lys-20'; however the relevance of this activity is unclear in vivo. TrxG protein are generally required to maintain the transcriptionally active state of homeotic genes throughout development. Does not act as a coactivator required for transcriptional activation, but specifically prevent inappropriate Polycomb Group (PcG) silencing of homeotic genes in cells in which they must stay transcriptionally active. Binds non-coding RNAs of trithorax response element (TRE), leading to its recruitment to the corresponding TRE in chromatin.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for ASH1 Antibody (50100002)(2)

Publications using 50100002

• Rybina OY, Rozovsky YM, Veselkina ER, Pasyukova EG. Polycomb/Trithorax group-dependent regulation of the neuronal gene Lim3 involved in Drosophila lifespan control Biochim. Biophys. Acta 2018-03-16 [PMID: 29555581] (Chemotaxis, Drosophila)
• Akan I, Love DC, Harwood KR et al. Drosophila O-GlcNAcase Deletion Globally Perturbs Chromatin O-GlcNAcylation. J Biol Chem 2016-05-06 [PMID: 26957542]

FAQs for ASH1 Antibody (50100002). (Showing 1 - 1 of 1 FAQs).

1. I am looking for an AB that was orig. created for the modENCODE project by SDIX and recognizes Drosophila ASH1. The modENCODE description of the AB can be found here Is your primary Ab ASH1 50100002 the one I am looking for? The product description on your webpage is very unclear, since your species assignment is Drosophila, but the gene symbol is human (ASH1L). There is also no quality information, like a Western blot nor any information on the source of that antibody or the antigen used for rabbit immunization. Please get me into contact with someone, who can give me background information on this antibody a.s.a.p.
   • Yes, 50100002 is the antibody you are looking for.

Bioinformatics

• Gene Symbol: ASH1L