ASB9 Antibody (1D8) - Azide and BSA Free

Western Blot: ASB9 Antibody (1D8) [H00140462-M01] - ASB9 monoclonal antibody (M01), clone 1D8 Analysis of ASB9 expression in HepG2.

Immunohistochemistry-Paraffin: ASB9 Antibody (1D8) [H00140462-M01] - Analysis of monoclonal antibody to ASB9 on formalin-fixed paraffin-embedded human lymphoma tissue. Antibody concentration 3 ug/ml.

Western Blot: ASB9 Antibody (1D8) [H00140462-M01] - Analysis of ASB9 expression in transfected 293T cell line by ASB9 monoclonal antibody (M01), clone 1D8.Lane 1: ASB9 transfected lysate(31.9 KDa).Lane 2: Non-transfected lysate.

Immunoprecipitation: ASB9 Antibody (1D8) [H00140462-M01] - Analysis of ASB9 transfected lysate using anti-ASB9 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with ASB9 MaxPab rabbit polyclonal antibody.

Sandwich ELISA: ASB9 Antibody (1D8) [H00140462-M01] - Detection limit for recombinant GST tagged ASB9 is approximately 0.1ng/ml as a capture antibody.

• Reactivity: Hu
• Applications: WB, ELISA, IHC, IP
• Clone: 1D8
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

ASB9 Antibody (1D8) - Azide and BSA Free Summary

• Immunogen: ASB9 (AAH01244, 1 a.a. ~ 252 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MDGKQGGMDGSKPAGPRDFPGIRLLSNPLMGDAVSDWSPMHEAAIHGHQLSLRNLISQGWAVNIITADHVSPLHEACLGGHLSCVKILLKHGAQVNGVTADWHTPLFNACVSGSWDCVNLLLQHGASVQPESDLASPIHEAARRAYGGNIDHKISHLGTPLYLACENQQRACVKKLLESGADVNQGKGQDSPLHAVARTASEELACLLMDFGADTQAKNAEGKRPVELVPPESPLAQLFLEREGASLPKPKP
• Specificity: ASB9 - ankyrin repeat and SOCS box-containing 9
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: ASB9
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Immunoprecipitation 1:10-1:500
  • Sandwich ELISA
  • Western Blot 1:500
• Application Notes: Antibody reactive against cell lysate and recombinant protein for Western Blot. Has also been used for immunohistochemistry (paraffin) and ELISA.

Reactivity Notes

Other species not tested.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for ASB9 Antibody (1D8) - Azide and BSA Free

• ankyrin repeat and SOCS box containing 9
• ankyrin repeat and SOCS box protein 9
• ankyrin repeat and SOCS box-containing 9
• ankyrin repeat and suppressor of cytokine signaling box protein 9
• ASB-9
• DKFZP564L0862
• FLJ20636
• MGC4954

Background

The ASB9 gene encodes a member of the ankyrin repeat and suppressor of cytokine signaling (SOCS) box protein family.Members of this family can interact with the elongin B-C adapter complex via their SOCS box domain and further complexwith the cullin and ring

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for ASB9 Antibody (H00140462-M01)(1)

Publication using H00140462-M01

• Kwon S, Kim D, Rhee JW et al. ASB9 interacts with ubiquitous mitochondrial creatine kinase and inhibits mitochondrial function. BMC Biol. 2010-03-19 [PMID: 20302626]

Bioinformatics

• Gene Symbol: ASB9
• Entrez:
  • 140462(Human)
• Uniprot:
  • AAH01244(Human)