RELT/TNFRSF19L Antibody [Unconjugated]

Western blot shows lysates of human bone marrow tissue and human lymph node tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human RELT/TNFRSF19L Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1385) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for RELT/TNFRSF19L at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

• Reactivity: Hu
• Applications: WB
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

RELT/TNFRSF19L Antibody [Unconjugated] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human RELT/TNFRSF19L
  Ser26-Ala160 (Arg127Gly, Arg129Gly)
  Accession # Q969Z4
• Specificity: Detects human RELT/TNFRSF19L in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human (rh) 4-1BB, rhBAFF R, rhCD27, rhTAJ, rhCD30, rhDR3, rhDR6, rhTNF RI, rhTNF RII, rhEDAR, rhFas, rhGITR, rhHVEM, rhNGF R, rhOPG, and recombinant mouse OX40 is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: RELT
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for RELT/TNFRSF19L Antibody [Unconjugated]

• Receptor expressed in lymphoid tissues
• RELT tumor necrosis factor receptor
• RELT
• TNFRSF19L
• TNFRSF19LFLJ14993
• tumor necrosis factor receptor superfamily member 19L
• tumor necrosis factor receptor superfamily, member 19-like

Background

RELT (Receptor Expressed in Lymphoid Tissues) is a type I transmembrane glycoprotein belonging to the tumor necrosis factor receptor superfamily (TNFRSF) and has been designated TNFRSF19-like (TNFRSF19L) (1, 2). It is primarily expressed in hematopoietic tissues and peripheral blood leukocytes. Human RELT cDNA encodes a 430 amino acid (aa) residue precursor protein with a putative 26 aa signal peptide, a 136 aa extracellular domain containing one TNF receptor cysteine-rich domain and one potential N-linked glycosylation site, a 21 aa transmembrane domain and a 247 aa cytoplasmic region containing no death domain. Human RELT shares 85% and 96% aa sequence homology with mouse RELT (Accession # BAC40459) and macaque RELT (Accession # Q9N092), respectively. Among TNFRSF members, the RELT extracellular domain is most closely related to that of TNFRSF19 and OX40. RELT has been shown to exclusively bind the adaptor protein TNF receptor-associated factor 1 (TRAF1). However, it has also been shown to activate the NF-kappa B pathway independently of TRAFs. Immobilized RELT can co‑stimulate T-cell proliferation in the presence of CD3 signaling, suggesting a potential regulatory role in immune response.

1. http://www.gene.ucl.ac.uk/nomenclature/genefamily/tnftop.html.
2. Sica, G. et al. (2001) Blood 97:2702.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for RELT/TNFRSF19L Antibody (AF1385)(5)

Publications using AF1385

• Batiste, M;Joy, B;Yee, CK;Cho, L;Christensen, A;Abed, I;Nguyen, K;Yanumula, A;Chang, H;Cho, ED;Wang, W;Chou, E;Chang, EH;Shyu, YL;Abram, A;Alcaide, J;Zhou, J;Gillespie, B;Senderovich, M;Cusick, GA;Le, AV;Hoang, F;Shi, Y;Mohamed, E;Cusick, JK; RELT Is Upregulated in Breast Cancer and Induces Death in Breast Cancer Cells Biomedicines 2024-11-22 [PMID: 39767574] (Western Blot, Human)
• JK Cusick, Y Alhomsy, S Wong, G Talbott, VN Uversky, C Hart, N Hejazi, AT Jacobs, Y Shi RELT stains prominently in B-cell lymphomas and binds the hematopoietic transcription factor MDFIC Biochemistry and Biophysics Reports, 2020-12-15;24(0):100868. 2020-12-15 [PMID: 33367115] (IHC, Human)
• Pachai Moua, Mathew Checketts, Liang-Guo Xu, Hong-Bing Shu, Mary E. Reyland, John K. Cusick RELT family members activate p38 and induce apoptosis by a mechanism distinct from TNFR1 Biochemical and Biophysical Research Communications 9/1/2017 [PMID: 28688764]
• Pachai Moua, Mathew Checketts, Liang-Guo Xu, Hong-Bing Shu, Mary E. Reyland, John K. Cusick RELT family members activate p38 and induce apoptosis by a mechanism distinct from TNFR1 Biochemical and Biophysical Research Communications 2017-09-01 [PMID: 28688764] (Western Blot, Human)
• Cusick JK, Mustian A, Goldberg K, Reyland ME RELT induces cellular death in HEK 293 epithelial cells. Cell. Immunol., 2009-11-04;261(1):1-8. 2009-11-04 [PMID: 19969290] (Western Blot, Human)

Bioinformatics

• Gene Symbol: RELT
• Uniprot:
  • Q969Z4()