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ASB13 Antibody

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Western Blot: ASB13 Antibody [NBP2-92275] - Analysis of extracts of mouse kidney, using ASB13 at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 10s.
Western Blot: ASB13 Antibody [NBP2-92275] - Analysis of extracts of mouse kidney, using ASB13 at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

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ASB13 Antibody Summary

Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 179-278 of human ASB13 (NP_078977.2).
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
ASB13
Purity
Affinity purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:2000

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS with 50% glycerol, pH7.3.
Preservative
0.02% Sodium Azide
Purity
Affinity purified

Alternate Names for ASB13 Antibody

  • ankyrin repeat and SOCS box containing 13
  • ankyrin repeat and SOCS box protein 13
  • ankyrin repeat and SOCS box-containing 13
  • ankyrin repeat domain-containing SOCS box protein Asb-13
  • ASB-13
  • FLJ13134
  • MGC19879

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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LMO2 Antibody
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AF2726
LMO2 was detected in immersion fixed K562 human chronic myelogenous leukemia cell line using Human LMO2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2726) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red, upper panel; Catalog # NL001) and counterstained with DAPI (blue, lower panel). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
K562 chronic myelogenous leukemia human cell line was stained with Goat Anti-Human LMO2 Antigen Affinity‑purified Polyclonal Antibody (Catalog # AF2726, filled histogram) or control antibody (Catalog # AB-108-C, open histogram), followed by Allophycocyanin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0108). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012). View our protocol for Staining Membrane-associated Proteins.
Human PBMC were stained with Goat Anti-Human LMO2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2726, filled histogram) or control antibody (Catalog # AB-108-C, open histogram), followed by Allophycocyanin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0108). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012). View our protocol for Staining Membrane-associated Proteins.
(7 Publications)
Species: Hu
Applications: Flow, ICC, WB
CDKN3 Antibody
(OTI2E11)
NBP2-01245
Flow Cytometry: CDKN3 Antibody (2E11) [NBP2-01245] - Analysis of Jurkat cells, using anti-CDKN3 antibody, (Red), compared to a nonspecific negative control antibody (Blue).
Flow Cytometry: CDKN3 Antibody (2E11) [NBP2-01245] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-CDKN3 antibody, and then analyzed by flow cytometry.
Western Blot: CDKN3 Antibody (2E11) [NBP2-01245] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CDKN3 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CDKN3.
Species: Hu, Mu
Applications: Flow, ICC/IF, IHC, IHC-P, WB
LRMP/Jaw1 Antibody
NBP1-87724
Western Blot: LRMP/Jaw1 Antibody [NBP1-87724] - Analysis in human cell line Daudi.
Immunohistochemistry-Paraffin: LRMP/Jaw1 Antibody [NBP1-87724] - Staining of human lymph node shows high expression.
Immunohistochemistry-Paraffin: LRMP/Jaw1 Antibody [NBP1-87724] - Staining of human skeletal muscle shows low expression as expected.
Species: Hu
Applications: IHC, IHC-P, WB
Cdk3 Antibody
(3C12)
H00001018-M01
Western Blot: Cdk3 Antibody (3C12) [H00001018-M01] - Analysis of CDK3 expression in transfected 293T cell line by CDK3 monoclonal antibody (M01), clone 3C12.Lane 1: CDK3 transfected lysate(35 KDa).Lane 2: Non-transfected lysate.
Immunohistochemistry-Paraffin: Cdk3 Antibody (3C12) [H00001018-M01] - Analysis of monoclonal antibody to CDK3 on formalin-fixed paraffin-embedded human testis. Antibody concentration 3 ug/ml.
Sandwich ELISA: Cdk3 Antibody (3C12) [H00001018-M01] - Detection limit for recombinant GST tagged CDK3 is approximately 0.1ng/ml as a capture antibody.
(1 Publication)
Species: Hu
Applications: ELISA, IHC, IHC-P, S-ELISA, WB
NEIL1 Antibody
NBP2-47358
Immunocytochemistry/Immunofluorescence: NEIL1 Antibody [NBP2-47358] - Immunofluorescent staining of human cell line HeLa shows localization to nucleoplasm. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: NEIL1 Antibody [NBP2-47358] - Staining of human cerebral cortex shows strong nuclear positivity in neurons.
Immunohistochemistry-Paraffin: NEIL1 Antibody [NBP2-47358] - Staining of human fallopian tube shows strong nuclear positivity in glandular cells.
Species: Hu
Applications: ICC/IF, IHC, IHC-P
CTGF/CCN2 Antibody
(88430) - C-terminus
MAB660
CTGF/CCN2 was detected in immersion fixed paraffin-embedded sections of human breast cancer tissue using 25 µg/mL Human CTGF/CCN2 C-Terminus Monoclonal Antibody (Catalog # MAB660) overnight at 4 °C. Tissue was stained with the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
(4 Reviews)
(6 Publications)
Species: Hu
Applications: IHC
A-Myb Antibody
NBP1-90171
Immunohistochemistry-Paraffin: A-Myb Antibody [NBP1-90171] - Staining of human testis shows moderate nuclear positivity in seminiferous ducts.
Immunohistochemistry-Paraffin: A-Myb Antibody [NBP1-90171] - Staining of human liver shows no nuclear positivity n hepatocytes as expected.
Immunohistochemistry-Paraffin: A-Myb Antibody [NBP1-90171] - Staining of human skeletal muscle shows no nuclear positivity in myocytes as expected.
(4 Publications)
Species: Hu, Mu
Applications: IHC, IHC-P, WB
FoxP1 Antibody
(JC12) - BSA Free
NB100-65125
Western Blot: FOXP1 Antibody (JC12) [NB100-65125] - Western blot analysis of resonicated MCF7 cell lysate (A) and MCF7 cell lysate (B) using FOXP1 antibody at 2 ug/ml.
Immunocytochemistry/Immunofluorescence: FOXP1 Antibody (JC12) [NB100-65125] - FOXP1 antibody was tested at 1:25 in MCF-7 cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red). Image objective 40x.
Flow Cytometry: FOXP1 Antibody (JC12) [NB100-65125] - Intracellular flow cytometric staining of 1 x 10^6 CHO (A) and HeLa (B) cells using FOXP1 antibody (dark blue). Isotype control shown in orange. An antibody concentration of 1 ug/1x10^6 cells was used.
(8 Publications)
Species: Hu, Mu
Applications: CyTOF-ready, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, WB
p18INK4c/CDKN2C
Antibody (18P118 (DCS ...
NB120-3216
Immunohistochemistry-Paraffin: p18INK4c/CDKN2C Antibody (18P118 (same as DCS-118)) [NB120-3216] - Human tonsil stained with p18 (NB120-3216) using peroxidase-conjugate and AEC chromogen. Note nuclear staining of cells.
Immunoprecipitation: p18INK4c/CDKN2C Antibody (18P118 (same as DCS-118)) [NB120-3216] - Analysis of native IP.
Western Blot: p18INK4c/CDKN2C Antibody (18P118 (same as DCS-118)) [NB120-3216] - Analysis of HeLa Cells.
(2 Publications)
Species: Hu, Mu
Applications: IF, IHC, IHC-P, IP, WB
Cyclin D2 Antibody
NBP3-13225
Western Blot: Cyclin D2 Antibody [NBP3-13225] - Whole cell extract (30 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with Cyclin D2 antibody (NBP3-13225) diluted at 1:1000.
Immunohistochemistry-Paraffin: Cyclin D2 Antibody [NBP3-13225] - Cyclin D2 antibody detects Cyclin D2 protein at cytoplasm in human cervical carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human cervical carcinoma. Cyclin D2 antibody (NBP3-13225) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Immunocytochemistry/Immunofluorescence: Cyclin D2 Antibody [NBP3-13225] - Cyclin D2 antibody detects Cyclin D2 protein at cytoplasm and nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Cyclin D2 protein stained by Cyclin D2 antibody (NBP3-13225) diluted at 1:500. Blue: Hoechst 33342 staining.
Species: Hu
Applications: ICC/IF, IHC, IHC-P, WB
MMP-12 Antibody
(82902)
MAB919
MMP-12 Antibody (82902)
(8 Publications)
Species: Hu
Applications: AP, IHC, IP, WB
ABCB6 Antibody
NBP2-58327
Western Blot: ABCB6 Antibody [NBP2-58327] - Analysis in human cell lines Caco-2 and A-431. Corresponding RNA-seq data are presented for the same cell lines. Loading control: Anti-PARP1.
Immunocytochemistry/Immunofluorescence: ABCB6 Antibody [NBP2-58327] - Staining of human cell line U-251 MG shows localization to nucleoplasm & the Golgi apparatus. Antibody staining is shown in green.
(1 Publication)
Species: Hu, Mu
Applications: ICC/IF, WB
Goat anti-Chicken
IgM Heavy Chain Secon ...
NB7276
Goat anti-Chicken IgM Heavy Chain Secondary Antibody
(37 Publications)
Species: Ch
Applications: ELISA, ICC/IF, IHC, IHC-P, WB

Glucosylceramidase/GBA
H00002629-M01
Western Blot: Glucosylceramidase/GBA Antibody (2E2) [H00002629-M01] - Analysis of GBA expression in transfected 293T cell line by GBA monoclonal antibody (M01), clone 2E2.Lane 1: GBA transfected lysate(60 KDa).Lane 2: Non-transfected lysate.
ELISA: Glucosylceramidase/GBA Antibody (2E2) [H00002629-M01] - Glucosylceramidase/GBA Antibody (2E2) [H00002629-M01] - Detection limit for recombinant GST tagged GBA is approximately 1ng/ml as a capture antibody.
Western Blot: Glucosylceramidase/GBA Antibody (2E2) [H00002629-M01] - Effect of Diltiazem on L444P and N370S/V394L GC Folding and Trafficking.Western blot analysis of untreated and diltiazem-treated L444P GC cells. L444P GC cells were cultured without or with diltiazem at varying concentrations for 7 d before the cells were lysed for SDS-PAGE and Western blot analysis. GC was detected using mouse anti-GC antibody 2E2. Beta-actin served as a loading control. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pbio.0060026) licensed under a CC-BY licence.
(7 Publications)
Species: Hu, Pl
Applications: ELISA, ICC/IF, IHC, IHC-P, IP, WB
Bcl-2 Antibody
NB100-56098
Western Blot: Bcl-2 Antibody [NB100-56098] - EA reduced MPP+-induced dopaminergic neuronal apoptosis by increasing BDNF (brain-derived neurotrophic factor) expression and further Akt phosphorylation in the rat substantia nigra. Eight days after MPP+ administration, our Western blot results (MAB7566) show that MPP+ treatment reduced tyrosine hydroxylase and Bcl-2 expression in the ipsilateral side of the rat substantia nigra (SN), but not in the contralateral side. EA stimulation (50 Hz) enhanced mature BDNF, tyrosine hydroxylase, and Bcl-2 expression in the MPP+-treated ipsilateral side. Image collected and cropped by CiteAb from the following publication (http://www.mdpi.com/1422-0067/18/9/1846), licensed under a CC-BY licence.
Western Blot: Bcl-2 Antibody [NB100-56098] - Analysis of Bcl-2 in whole cell lysate from Daoy cells. Cells were transfected with (1) scrambled control siRNA or (2) Bcl-2 siRNA. Image from verified customer review.
Western Blot: Bcl-2 Antibody [NB100-56098] - Analysis of Bcl-2 using this antibody at 1:2000, 20 ug/protein was loaded per lane. (1) Human Jurkat T cells. (2) Human RS11 lymphoma cells. (3-6) Human breast cancer cases. Patient sample in lane 5 lacked detectible Bcl-2 expression.
(13 Publications)
Species: Ca, Hu, Mu, Rt
Applications: IHC, IHC-P, IP, WB
IRF4 Antibody
AF5525
IRF4 was detected in immersion fixed paraffin-embedded sections of human tonsil using Sheep Anti-Human IRF4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5525) at 3 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm of lymphocytes. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Western blot shows lysates of Ramos human Burkitt's lymphoma cell line, Raji human Burkitt's lymphoma cell line, and RPMI 8226 human multiple myeloma cell line. PVDF membrane was probed with 0.5 µg/mL of Sheep Anti-Human IRF4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5525) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for IRF4 at approximately 53 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
(1 Publication)
Species: Hu
Applications: IHC, WB
JNK3 Antibody
(SD082-09)
NBP2-67800
Flow Cytometry: JNK3 Antibody (SD082-09) [NBP2-67800] - Analysis of Jurkat cells with JNK3 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
Immunocytochemistry/Immunofluorescence: JNK3 Antibody (SD082-09) [NBP2-67800] - Staining JNK3 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Immunocytochemistry/Immunofluorescence: JNK3 Antibody (SD082-09) [NBP2-67800] - Staining JNK3 in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Species: Hu, Mu, Rt
Applications: Flow, ICC/IF, WB
Neprilysin/CD10
Antibody [Unconjugate
AF1126
Neprilysin/CD10 was detected in perfusion fixed frozen sections of mouse brain (glial cell in hippocampus) using 15 µg/mL Goat Anti-Mouse Neprilysin/CD10 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1126) overnight at 4 °C. Tissue was stained (red) and counterstained (green). View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.
(2 Reviews)
(7 Publications)
Species: Mu
Applications: ELISA(Cap), ELISA(Det), ELISA(Sta), IHC, IP, WB
Collagen III alpha
1/COL3A1 Antibody
NB600-594
Immunohistochemistry: Collagen III alpha 1/COL3A1 Antibody [NB600-594] - Staining in FFPE sections of human skin(left, dermis) with moderate to strong red staining and testis (right) where strong staining was observed within connective tissue between seminiferous tubules. The antibody showed strong extracellular staining within connective tissues across many organs with minimal background staining. Slides were steamed in 0.01 M sodium citrate buffer, pH 6.0 at 99-100C. 20 minutes for antigen retrieval.
Immunohistochemistry: Collagen III alpha 1/COL3A1 Antibody [NB600-594] - Tissue: right lobe of the liver section. A:Central Vein (CV) fibrosis, B: Non-fibrotic CV, C: Perisinusodial fibrosis, D: Non-fibrotic area, E: Protat tract fibrosis, F: Septal fibrosis (arrow). Fixation: FFPE. Antigen retrieval: not required. Primary antibody: Anti-collagen type I at 1:500 for 4 degrees Celsius for 24hr. Secondary antibody: Peroxidase biotin-streptavidin rabbit secondary antibody at 1:10,000 for 45 min at RT. Localization: Anti-collagen type III is intra and extracellular. Staining: 3.3'-diaminobenzidine tetrahydrochloride was used as the chromogen. Nuclei were counterstained purple with hematoxylin.
Western Blot: Collagen III alpha 1/COL3A1 Antibody [NB600-594] - Western blot for COL3A1 protein with PVDF protein staining to control for protein loading. Image collected and cropped by CiteAb from the following publication (mdpi.com/2076-3921/6/4/87), licensed under a CC-BY licence.
(32 Publications)
Species: Bv, Fe, Hu, Mu, Rt, Sh
Applications: ELISA, Flow, ICC/IF, IHC, IHC-P, IP, Simple Western, WB
Fibronectin
Antibody - BSA Free
NBP1-91258
Immunohistochemistry-Frozen: Fibronectin Antibody [NBP1-91258] - Feline corneal stromal cells. IHC-Fr and IF (Alexa Fluor488) were performed using NBP1-91258 at 1:400. Image captured by epifluorescent microscopy. Image from verified customer review.
Western Blot: Fibronectin Antibody [NBP1-91258] - Analysis of Fibronectin in HepG2 cell lysate.
Immunohistochemistry-Frozen: Fibronectin Antibody [NBP1-91258] - Trabecular meshwork (TM) region of pig eyes. NBP1-91258 Fibronectin antibody was labelled with Alexa Fluor 488 conjugated secondary antibody (Green). DAPI shown as blue. Image from verified customer review.
(38 Publications)
Species: Bv, Ca, Eq, Fe, Hu, Mu, Po, Rt
Applications: ICC/IF, IHC, IHC-Fr, IHC-P, Simple Western, WB

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Bioinformatics

Gene Symbol ASB13