ASAHL/N-acylethanolamine-hydrolyzing Acid Amidase Antibody

• Reactivity: Hu
• Applications: WB, IP
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

ASAHL/N-acylethanolamine-hydrolyzing Acid Amidase Antibody Summary

• Immunogen: Chinese hamster ovary cell line CHO-derived recombinant human ASAHL/N‑acylethanolamine-hydrolyzing Acid A isoform 1
  Ser29-Lys359 (Val151Ile)
  Accession # Q02083
• Specificity: Detects human ASAHL/N‑acylethanolamine-hydrolyzing Acid A in direct ELISAs and Western blots. In Western blots, approximately 25% cross‑reactivity with recombinant mouse ASAHL is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: NAAA
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation 25 ug/mL
  • Western Blot 0.1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for ASAHL/N-acylethanolamine-hydrolyzing Acid Amidase Antibody

• Acid ceramidase-like protein
• ASAHL
• ASAH-like protein
• EC 3.5.1.-
• NAAA
• N-acylethanolamine acid amidase
• Nacylethanolaminehydrolyzing Acid Amidase
• N-acylethanolamine-hydrolyzing acid amidase
• N-acylsphingosine amidohydrolase (acid ceramidase)-like
• N-acylsphingosine amidohydrolase-like
• PLT

Background

ASAHL (N-acylsphingosine-amidohydrolase-like; N acylethanolamine hydrolyzine acid amidase; also acid ceramidase like protein) is a 30 kDa member of the acid ceramidase family. It is a secreted lysosomal glycoprotein that cleaves fatty acid amides such as palmitoylethanolamine. Human ASAHL is synthesized as a 359 amino acid (aa) preproprecursor that contains a 28 aa signal sequence, a 96 aa prosegment, and a 30 31 kDa, 235 aa mature region that begins at Cys125. Multiple isoforms likely exist. One isoform shows a deletion of aa 324 359, while another has a two aa substitution for aa 198 359. The human proform (aa 29 359) is 78% aa identical to mouse ASAHL proform.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for ASAHL/N-acylethanolamine-hydrolyzing Acid Amidase Antibody (AF4494)(1)

Publication using AF4494

• Suarez J, Romero-Zerbo Y, Marquez L, Rivera P, Iglesias M, Bermudez-Silva FJ, Andreu M, Rodriguez de Fonseca F Ulcerative colitis impairs the acylethanolamide-based anti-inflammatory system reversal by 5-aminosalicylic acid and glucocorticoids. PLoS ONE, 2012-05-25;7(5):e37729. 2012-05-25 [PMID: 22662201] (IHC, Western Blot, Human)

Bioinformatics

• Gene Symbol: NAAA
• Uniprot:
  • Q02083()