ASAH1 Overexpression Lysate

Product Discontinued

ASAH1 Overexpression Lysate Summary

• Immunogen: The lysate was created in HEK293T cells, using plasmid ID RC215316 and based on accession number NP_808592. The protein contains a C-terminal DDK tag.
• Specificity: Homo sapiens ASAH1, mRNA.
• Gene: ASAH1

Applications/Dilutions

• Dilutions:
  • Western Blot
• Application Notes: This product is intended for use as a positive control in Western Blot.
  
  You will receive 1 vial of lysate (100ug), 1 vial of empty vector negative control (100ug), and 1 vial of 2xSDS sample buffer (250ul).
• Theoretical MW: 42.6 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -80C. Avoid freeze-thaw cycles.
• Buffer: RIPA buffer

Lysate Details for Array

• Type: Overexpression

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for ASAH1 Overexpression Lysate

• ACDase
• ACEC 3.5.1.23
• Acid CDase
• acid ceramidase
• Acylsphingosine deacylase
• ASAH
• FLJ21558
• FLJ22079
• N-acylsphingosine amidohydrolase (acid ceramidase) 1
• N-acylsphingosine amidohydrolase
• PHP
• PHP32N-acylsphingosine amidohydrolase (acid ceramidase)
• Putative 32 kDa heart protein

Background

ASAH1 encodes a heterodimeric protein consisting of a nonglycosylated alpha subunit and a glycosylated beta subunit that is cleaved to the mature enzyme posttranslationally. The encoded protein catalyzes the synthesis and degradation of ceramide into sphingosine and fatty acid. Mutations in this gene have been associated with a lysosomal storage disorder known as Farber disease. Multiple transcript variants encoding several distinct isoforms have been identified for this gene. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Bioinformatics

• Gene Symbol: ASAH1