ARPC3 Antibody

Western Blot: ARPC3 Antibody [NB300-895] - (1ug/ml) of Mouse Brain lysate (RIPA buffer, 35ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

• Reactivity: Hu, Mu, Rt, Bv, Ca
• Applications: WB, ELISA
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

ARPC3 Antibody Summary

• Description: Novus Biologicals Goat ARPC3 Antibody (NB300-895) is a polyclonal antibody validated for use in WB and ELISA. Anti-ARPC3 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Peptide with sequence C-RQFMNKSLSGPGQ corresponding to C-Terminus according to NP_005710.1.
• Specificity: Please note there are two hypothetical proteins called "similar to the Arp2/3 protein complex subunit p21-Arc" that are virtually identical (CAC14083, XP_208062).
• Predicted Species: Rat (100%), Canine (100%), Bovine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: ARPC3
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Peptide ELISA Detection limit 1:16000
  • Western Blot 1 - 3 ug/ml
• Application Notes: WB: Band observed just below 20 kDa band marker in Hela, human brain and mouse brain lysates (predicted MW of 20 kDa band according to NP_005710).

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for ARPC3 Antibody

• actin related protein 2/3 complex, subunit 3, 21kDa
• subunit 3 (21 kD)

Background

This gene encodes one of seven subunits of the human Arp2/3 protein complex. The Arp2/3 protein complex has been implicated in the control of actin polymerization in cells and has been conserved through evolution. The exact role of the protein encoded by this gene, the p21 subunit, has yet to be determined.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for ARPC3 Antibody (NB300-895)(1)

Publication using NB300-895

• Welch MD, DePace AH, Verma S et al. The human Arp2/3 complex is composed of evolutionarily conserved subunits and is localized to cellular regions of dynamic actin filament assembly. J Cell Biol 1997-07-28 [PMID: 9230079]

Bioinformatics

• Gene Symbol: ARPC3
• Entrez:
  • 10094(Human)
  • 56378(Mouse)
• Uniprot:
  • O15145(Human)