Rat ARNT2 ELISA Kit (Colorimetric)

ELISA: Rat ARNT2 ELISA Kit (Colorimetric) [NBP2-69862] - Samples were spiked with high concentrations of Rat ARNT2 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

ELISA: Rat ARNT2 ELISA Kit (Colorimetric) [NBP2-69862] - Standard Curve Reference

• Reactivity: Rt
• Applications: ELISA
• Suitable Sample Type: Serum, plasma and other biological fluids
• Standard Curve Range: 0.16 - 10 ng/mL
• Sensitivity: 0.10 ng/mL

Rat ARNT2 ELISA Kit (Colorimetric) Summary

• Specificity: This kit recognizes Rat ARNT2 in samples. No significant cross-reactivity or interference between Rat ARNT2 and analogues was observed.
• Standard Curve Range: 0.16 - 10 ng/mL
• Sensitivity: 0.10 ng/mL
• Assay Type: Sandwich-ELISA
• Inter-Assay: CV% < 5%
• Intra-Assay: CV% < 4.67%
• Spike Recovery: 88-108%
• Sample Volume: 100 uL
• Kit Type: ELISA Kit (Colorimetric)
• Gene: ARNT2

Applications/Dilutions

• Dilutions:
  • ELISA

Packaging, Storage & Formulations

• Storage: Storage of components varies. See protocol for specific instructions.

Alternate Names for Rat ARNT2 ELISA Kit (Colorimetric)

• ARNT protein 2
• aryl-hydrocarbon receptor nuclear translocator 2
• bHLHe1BHLHE1
• Class E basic helix-loop-helix protein 1
• KIAA0307aryl hydrocarbon receptor nuclear translocator 2

Background

AhR, Arnt 1, Arnt 2 and BMAL1 are members of a family of transcription factors that contain a basic helix-loop-helix motif and a common "PAS" motif. The aromatic (aryl) hydrocarbon receptor, AhR, is a ligand dependent transcription factor that interacts with specific DNA sequences termed xenobiotic responsive elements (XREs) to activate several genes including CYP1A1, glutathione S-transferase Ya subunit and DT-diaphorase. The Ah receptor nuclear translocator proteins (Arnt 1 or Arnt 2) are required for ligand-dependent nuclear translocation of the Ah receptor and are also necessary for Ah receptor binding to the XRE element. BMAL1 (Brain and Muscle Arnt-like protein 1), also designated Arnt 3, TIC, JAP3 or MOP-3, has been shown to dimerize with Clock and bind to the promoter region of mPer1, suggesting that this protein plays a role in regulation of circadian oscillation in mammals.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

Bioinformatics

• Gene Symbol: ARNT2