ARHGEF4 Antibody

Western Blot: ARHGEF4 Antibody [NB100-1316] - Analysis of ARHGEF4 in Human Brain lysate using this antibody at 2 ug/ ml.

Western Blot: ARHGEF4 Antibody [NB100-1316] - HEK293 overexpressing ARHGEF4 (RC215591) and probed with this antibody (mock transfection in first lane).

• Reactivity: Hu
• Applications: WB, ELISA
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

ARHGEF4 Antibody Summary

• Immunogen: Peptide with sequence PWEEPAGEKPSCS corresponding to N-Terminus according to NP_056135.2, NP_127462.1.
• Specificity: This antibody is expected to recognise both reported isoforms (NP_056135.2; NP_127462.1).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: ARHGEF4
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Peptide ELISA Detection limit 1:32000
  • Western Blot 2 - 4 ug/ml
• Application Notes: WB: Approx. 80 kDa band observed in human brain lysates and in transfected HEK293 transiently expressing ARHGEF4 (calculated MW of 79.1 kDa band according to NP_056135.2 and 76.4 kDa band according to 127462.1).
• Theoretical MW: 79 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control:

  ARHGEF4 Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for ARHGEF4 Antibody

• APC-stimulated guanine nucleotide exchange factor 1
• Asef
• ASEF1
• ASEFAPC-stimulated guanine nucleotide exchange factor
• GEF4
• KIAA1112
• Rho guanine nucleotide exchange factor (GEF) 4
• rho guanine nucleotide exchange factor 4
• STM6

Background

Rho GTPases play a fundamental role in numerous cellular processes that are initiated by extracellular stimuli that work through Gucleotide exchange protein coupled receptors. Acts as guanine nucleotide exchange factor (GEF) for RhoA and RAC1 GTPases. Binding of APC may activate RAC1 GEF activity. The APC-ARHGEF4 complex seems to be involved in cell migration as well as in E-cadherin-mediated cell-cell adhesion. This protein is similar to rat collybistin protein. Alternative splicing of the gene generates two transcript variants which encode different isoforms.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for ARHGEF4 Antibody (NB100-1316)(2)

Publications using NB100-1316

• Furukawa S, Kawasaki Y, Miyamoto M et al. The miR-1-NOTCH3-Asef Pathway Is Important for Colorectal Tumor Cell Migration. PLoS One. 2013-11-11 [PMID: 24244701] (WB, Human)
• Thiesen S, Kubart S, Ropers HH, Nothwang HG. Isolation of two novel human RhoGEFs, ARHGEF3 and ARHGEF4, in 3p13-21 and 2q22. Biochem Biophys Res Commun 2000-06-24 [PMID: 10873612]

Bioinformatics

• Gene Symbol: ARHGEF4
• Entrez:
  • 50649(Human)
• Uniprot:
  • Q9NR80(Human)