ARHGEF4 Antibody

Images

 
Western Blot: ARHGEF4 Antibody [NB100-1316] - Analysis of ARHGEF4 in Human Brain lysate using this antibody at 2 ug/ ml.
Western Blot: ARHGEF4 Antibody [NB100-1316] - HEK293 overexpressing ARHGEF4 (RC215591) and probed with this antibody (mock transfection in first lane).

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, PEP-ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

ARHGEF4 Antibody Summary

Immunogen
Peptide with sequence PWEEPAGEKPSCS corresponding to N-Terminus according to NP_056135.2, NP_127462.1.
Specificity
This antibody is expected to recognise both reported isoforms (NP_056135.2; NP_127462.1).
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
ARHGEF4
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Peptide ELISA Detection limit 1:32000
  • Western Blot 2 - 4 ug/ml
Application Notes
WB: Approx. 80 kDa band observed in human brain lysates and in transfected HEK293 transiently expressing ARHGEF4 (calculated MW of 79.1 kDa band according to NP_056135.2 and 76.4 kDa band according to 127462.1).
Theoretical MW
79 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control
ARHGEF4 Overexpression Lysate
Publications
Read Publications using
NB100-1316 in the following applications:

  • WB
    1 publication

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for ARHGEF4 Antibody

  • APC-stimulated guanine nucleotide exchange factor 1
  • Asef
  • ASEF1
  • ASEFAPC-stimulated guanine nucleotide exchange factor
  • GEF4
  • KIAA1112
  • Rho guanine nucleotide exchange factor (GEF) 4
  • rho guanine nucleotide exchange factor 4
  • STM6

Background

Rho GTPases play a fundamental role in numerous cellular processes that are initiated by extracellular stimuli that work through Gucleotide exchange protein coupled receptors. Acts as guanine nucleotide exchange factor (GEF) for RhoA and RAC1 GTPases. Binding of APC may activate RAC1 GEF activity. The APC-ARHGEF4 complex seems to be involved in cell migration as well as in E-cadherin-mediated cell-cell adhesion. This protein is similar to rat collybistin protein. Alternative splicing of the gene generates two transcript variants which encode different isoforms.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

NB100-91662
Species: Hu, Mu, Rt
Applications: Flow, ICC/IF, IHC, IHC-P, WB
NB110-68800
Species: Hu, Mu
Applications: ICC/IF, WB
NBP2-21577
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-Fr, IHC-P, IP, KD, WB
H00056731-M02
Species: Hu
Applications: ELISA, ICC/IF, WB
AF6260
Species: Hu
Applications: WB
NBP2-67895
Species: Hu, Mu, Rt
Applications: Flow, IHC, IHC-P, IP, WB
NBP1-85727
Species: Hu
Applications: ICC/IF, IHC, IHC-P
NBP2-66987
Species: Hu
Applications: ICC/IF, IHC, IHC-P, WB
NBP1-54467
Species: Ch, Av-Du, Hu, Mu, Pm, Rt
Applications: Flow-IC, Flow, ICC/IF, IHC, IHC-P, IP, Simple Western, WB
NBP1-30981
Species: Hu, Mu
Applications: ICC/IF, WB
AF2685
Species: Hu
Applications: IHC, Simple Western, WB
NB100-91266
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-Fr, IHC-P, WB
NB100-1343
Species: Hu
Applications: Flow, ICC/IF, IHC, IHC-P, PEP-ELISA, WB
294-HG/CF
Species: Hu
Applications: BA
NBP2-48615
Species: Hu
Applications: IHC, IHC-P, WB
NBP1-31234
Species: Hu
Applications: ICC/IF, IHC, IHC-P, IP, WB
233-FB/CF
Species: Hu
Applications: BA
NB100-91273
Species: Hu, Mu, Pm, Rt
Applications: ICC/IF, IHC, IHC-P, WB

Publications for ARHGEF4 Antibody (NB100-1316)(2)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: WB.


Filter By Application
WB
(1)
All Applications
Filter By Species
Human
(1)
All Species

Reviews for ARHGEF4 Antibody (NB100-1316) (0)

There are no reviews for ARHGEF4 Antibody (NB100-1316). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for ARHGEF4 Antibody (NB100-1316) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Additional ARHGEF4 Products

Bioinformatics Tool for ARHGEF4 Antibody (NB100-1316)

Discover related pathways, diseases and genes to ARHGEF4 Antibody (NB100-1316). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for ARHGEF4 Antibody (NB100-1316)

Discover more about diseases related to ARHGEF4 Antibody (NB100-1316).

Research Areas for ARHGEF4 Antibody (NB100-1316)

Find related products by research area.

Blogs on ARHGEF4

There are no specific blogs for ARHGEF4, but you can read our latest blog posts.
Omicron Variant Antibodies

Customers Who Bought This Also Bought

Contact Information

Download our Western Blot eHandbook

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our ARHGEF4 Antibody and receive a gift card or discount.

Bioinformatics

Gene Symbol ARHGEF4
Entrez
Uniprot