ARHGEF1 Antibody - BSA Free

Western Blot: ARHGEF1 Antibody [NBP2-56320] - Analysis using Anti-ARHGEF1 antibody NBP2-56320 (A) shows similar pattern to independent antibody NBP1-82843 (B).

Immunocytochemistry/Immunofluorescence: ARHGEF1 Antibody [NBP2-56320] - Staining of human cell line A549 shows localization to cytosol.

Analysis using NBP2-56320 (A) shows similar pattern to independent antibody NBP1-82843 (B).

• Reactivity: Hu
• Applications: WB, ICC/IF
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

ARHGEF1 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit ARHGEF1 Antibody - BSA Free (NBP2-56320) is a polyclonal antibody validated for use in WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: This antibody was developed against a recombinant protein corresponding to the following amino acid sequence: LRVPVPPNVAFELDRTRADLISEDVQRRFVQEVVQSQQVAVGRQLEDFRSKRLMGMTPWEQELAQLEAWVGRDRASYEARERHVAERLLMHLEEMQHTISTDEEKSAAVVNAIGL
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: ARHGEF1
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Western Blot 1:100 - 1:250
• Application Notes: Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.
• Control Peptide:

  ARHGEF1 Recombinant Protein Antigen (NBP2-56320PEP)

Reactivity Notes

Mouse 86%, Rat 87%

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Affinity purified

Alternate Names for ARHGEF1 Antibody - BSA Free

• 115 kDa guanine nucleotide exchange factor
• 115-kD protein
• GEF1
• LBCL2LSC
• Lsc homolog
• p115RhoGEF
• P115-RHOGEF
• Rho guanine nucleotide exchange factor (GEF) 1
• rho guanine nucleotide exchange factor 1
• SUB1.5

Background

Rho GTPases play a fundamental role in numerous cellular processes that are initiated by extracellular stimuli that work through G protein coupled receptors. The encoded protein may form complex with G proteins and stimulate Rho-dependent signals. Multiple alternatively spliced transcript variants have been found for this gene, but the full-length nature of some variants has not been defined.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ARHGEF1