ARHGAP25 Antibody - BSA Free

Western Blot: ARHGAP25 Antibody [NBP1-83053] - Staining of human lymph node.

Immunohistochemistry-Paraffin: ARHGAP25 Antibody [NBP1-83053] - Staining of human cerebral cortex using Anti-ARHGAP25 antibody NBP1-83053.

Western Blot: ARHGAP25 Antibody [NBP1-83053] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp. Lane 4: Human plasma (IgG/HSA depleted). Lane 5: Human liver tissue. Lane 6: Human tonsil tissue

Western Blot: ARHGAP25 Antibody [NBP1-83053] - Analysis using Anti-ARHGAP25 antibody NBP1-83053 (A) shows similar pattern to independent antibody NBP2-57228 (B).

Immunohistochemistry-Paraffin: ARHGAP25 Antibody [NBP1-83053] - Staining of human tonsil shows strong cytoplasmic positivity in lymphoid cells outside reaction centra.

Immunohistochemistry-Paraffin: ARHGAP25 Antibody [NBP1-83053] - Staining of human cerebral cortex shows low expression as expected.

Immunohistochemistry-Paraffin: ARHGAP25 Antibody [NBP1-83053] - Staining of human spleen shows high expression.

Immunohistochemistry-Paraffin: ARHGAP25 Antibody [NBP1-83053] - Staining in human spleen and cerebral cortex tissues using anti-ARHGAP25 antibody. Corresponding ARHGAP25 RNA-seq data are presented for the same tissues.

Immunohistochemistry-Paraffin: ARHGAP25 Antibody [NBP1-83053] - Staining of human cerebral cortex, colon, kidney and lymph node using Anti-ARHGAP25 antibody NBP1-83053 (A) shows similar protein distribution across tissues to independent antibody NBP2-57228 (B).

Immunohistochemistry-Paraffin: ARHGAP25 Antibody [NBP1-83053] - Staining of human kidney.

Immunohistochemistry-Paraffin: ARHGAP25 Antibody [NBP1-83053] - Staining of human colon.

• Reactivity: Hu
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

ARHGAP25 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit ARHGAP25 Antibody - BSA Free (NBP1-83053) is a polyclonal antibody validated for use in IHC and WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: This antibody was developed against Recombinant Protein corresponding to amino acids: IQRVMTMMIRDHEVLFPKSKDIPLSPPAQKNDPKKAPVARSSVGWDATEDLRISRTDSFSSMTSDSDTTSPTGQQPSDAFPEDSSKVPREK
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: ARHGAP25
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:200 - 1:500
  • Immunohistochemistry-Paraffin 1:200 - 1:500
  • Western Blot 0.04-0.4 ug/ml
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended.
• Theoretical MW: 73 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control Peptide:

  ARHGAP25 Protein (NBP1-83053PEP)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for ARHGAP25 Antibody - BSA Free

• KAIA0053
• KIAA0053
• Rho GTPase activating protein 25
• rho GTPase-activating protein 25
• Rho-type GTPase-activating protein 25

Background

ARHGAPs, such as ARHGAP25, encode negative regulators of Rho GTPases (see ARHA; MIM 165390), which are implicated in actin remodeling, cell polarity, and cell migration (Katoh and Katoh, 2004 [PubMed 15254788]).[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ARHGAP25
• Entrez:
  • 9938(Human)
• Uniprot:
  • P42331(Human)