ARFIP2 Antibody - BSA Free

Western Blot: ARFIP2 Antibody [NBP1-33195] - Sample (30 ug of whole cell lysate) A: Molt-4 10% SDS PAGE; antibody diluted at 1:10000.

Immunohistochemistry-Paraffin: ARFIP2 Antibody [NBP1-33195] - Paraffin-embedded human cervical cancer. ARFIP2 antibody [N1C2] diluted at 1:500.

Immunohistochemistry-Paraffin: ARFIP2 Antibody [NBP1-33195] - Paraffin-embedded human endometrial cancer. ARFIP2 antibody [N1C2] diluted at 1:500.

• Reactivity: Hu, Mu, Rt, Bv, Sh
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

ARFIP2 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit ARFIP2 Antibody - BSA Free (NBP1-33195) is a polyclonal antibody validated for use in IHC and WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant protein encompassing a sequence within the center region of human ARFIP2. The exact sequence is proprietary.
• Predicted Species: Mouse (99%), Rat (99%), Sheep (98%), Bovine (98%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: ARFIP2
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:5000-1:20000
• Theoretical MW: 38 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: 0.1M Tris, 0.1M Glycine, 10% Glycerol
• Preservative: 0.01% Thimerosal
• Purity: Antigen Affinity-purified

Alternate Names for ARFIP2 Antibody - BSA Free

• ADP-ribosylation factor interacting protein 2
• ADP-ribosylation factor-interacting protein 2
• arfaptin 2
• arfaptin-2
• Partner of RAC1
• POR1partner of RAC1 (arfaptin 2)
• Protein POR1

Background

ARFIP2 is a ubiquitously expressed protein implicated in mediating cross talk between RAC and ARF small GTPases. It has been shown that ARFIP2 binds specifically to GTP-bound ARF1 and ARF6, but binds to Rac-GTP and Rac-GDP with similar affinities. The X-ray structure of arfaptin reveals an elongated, crescent-shaped dimer of 3-helix coiled-coils. Structures of arfaptin with Rac bound to either GDP or the slowly hydrolysable analog GMPPNP show that the switch regions adopt similar conformations in both complexes.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Bioinformatics

• Gene Symbol: ARFIP2