Human APRT ELISA Kit (Colorimetric)

ELISA: Human APRT ELISA Kit (Colorimetric) [NBP3-18695] - Standard Reference Curve

• Reactivity: Hu
• Applications: ELISA
• Suitable Sample Type: Serum, Plasma, Cell Culture, Tissue Extract
• Standard Curve Range: 0.075 - 4.8 ng/mL (example only; lot dependent)
• Sensitivity: 25 pg/mL (example only; lot dependent)

Human APRT ELISA Kit (Colorimetric) Summary

• Description: This assay employs a quantitative enzyme immunoassay technique that measures the specified antigen in samples.
• Standard Curve Range: 0.075 - 4.8 ng/mL (example only; lot dependent)
• Sensitivity: 25 pg/mL (example only; lot dependent)
• Assay Type: Sandwich ELISA
• Inter-Assay: CV%< 10.7 (example only; lot dependent)
• Intra-Assay: CV%< 6.3 (example only; lot dependent)
• Spike Recovery: 101% (example only; lot dependent)
• Kit Type: ELISA Kit (Colorimetric)
• Gene: APRT

Applications/Dilutions

• Dilutions:
  • ELISA
  • Sandwich ELISA

Packaging, Storage & Formulations

• Storage: Storage of components varies. See protocol for specific instructions.

Alternate Names for Human APRT ELISA Kit (Colorimetric)

• adenine phosphoribosyltransferase
• AMP diphosphorylase
• AMP pyrophosphorylase
• AMP
• DKFZp686D13177
• EC 2.4.2.7
• MGC125856
• MGC125857
• MGC129961
• transphosphoribosidase

Background

Adenine phosphoribosyltransferase belongs to the purine/pyrimidine phosphoribosyltransferase family. A conserved feature of this gene is the distribution of CpG dinucleotides. This enzyme catalyzes the formation of AMP and inorganic pyrophosphate from adenine and 5-phosphoribosyl-1-pyrophosphate (PRPP). It also produces adenine as a by-product of the polyamine biosynthesis pathway. A homozygous deficiency in this enzyme causes 2,8-dihydroxyadenine urolithiasis. Two transcript variants encoding different isoforms have been found for this gene.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

FAQs for APRT ELISA Kit (NBP3-18695). (Showing 1 - 1 of 1 FAQ).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

Bioinformatics

• Gene Symbol: APRT