APP 695+1 Antibody [Unconjugated]

APP695+1was detected in immersion fixed paraffin-embedded sections of human Alzheimer's brain using Goat Anti-Human APP695+1Antigen Affinity-purified Polyclonal Antibody (Catalog # AF890) at 10 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

• Reactivity: Hu
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

APP 695+1 Antibody [Unconjugated] Summary

• Immunogen: S. frugiperda insect ovarian cell line Sf 21-derived recombinant human APP695⁺¹ frameshift mutant
  Met1-Arg336
  Accession # NP_958817
• Specificity: Detects human APP695⁺¹ in direct ELISAs and Western blots.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: APP
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 5-15 ug/mL
  • Western Blot 0.1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for APP 695+1 Antibody [Unconjugated]

• AAA;ABETA;ABPP;AD1;alpha-sAPP;Amyloid-beta A4 protein;Amyloid-beta precursor protein;APPI;CTFgamma;CVAP;PN2;PN-II;preA4
• APP695+1

Background

Amyloid precursor protein (APP) is a type I membrane protein with several human isoforms due to alternative splicing. APP770, 751, and 733 contain a Kunitz protease inhibitor (KPI) domain (residue 291‑342) and APP695 does not. APP is a cell surface molecule with many functions. It can be processed proteolytically in two different pathways. In one pathway, beta - and gamma -secretase cleave at the  beta  site between residue 670 and 671 and the gamma site between residue 711 and 714 to produce beta ‑amyloid peptide (A beta 40 and A beta 42), a major component in plaques found in brains of patients with Alzheimer's disease (1). The other pathway involves alpha -secretase that cleaves residues between 687 and 688. It is antiamyloidogenic due to its benign character and the prevention of the A beta peptide formation (2). Soluble APP containing the KPI domain, also referred to as protease nexin II, is a potent inhibitor of serine proteases and may have additional functions. For example, it may regulate the contact face of blood coagulation and limit thrombosis specially in brain due to its localization and coagulation factor XI inhibiting activity (3, 4).

1. Haass, C. (2004) EMBO J. 23:483.
2. Lichtenthaler, S. F. and C. Haass (2004) J. Clin. Invest. 113:1384.
3. Badellino, K.O. and P.N. Walsh (2000) Biochemistry 39:4769.
4. Xu, F. et al. (2005) Proc. Natl. Acad. Sci USA. 102:18135.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Review for APP 695+1 Antibody (AF890) (1)

Average Rating: 3

(Based on 1 review)

We have 1 review tested in 1 species: Mouse.

reviewed by: Verified Customer IF Mouse 12/19/2014

Summary

• Application: Immunofluorescence
• Sample Tested: Formaldehyde-fixed P0 mouse hippocampal neurons
• Species: Mouse

Bioinformatics

• Gene Symbol: APP
• Uniprot:
  • NP_958817()