Apolipoprotein M/ApoM Antibody (1F10) - Azide and BSA Free

Western Blot: Apolipoprotein M/ApoM Antibody (1F10) [H00055937-M01] - Analysis of APOM expression in transfected 293T cell line by APOM monoclonal antibody (M01), clone 1F10.Lane 1: APOM transfected lysate(21.3 KDa).Lane 2: Non-transfected lysate.

Western Blot: Apolipoprotein M/ApoM Antibody (1F10) [H00055937-M01] - Analysis of APOM over-expressed 293 cell line, cotransfected with APOM Validated Chimera RNAi ( Cat # H00055937-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with APOM monoclonal antibody (M01), clone 1F10 (Cat # H00055937-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.

Sandwich ELISA: Apolipoprotein M/ApoM Antibody (1F10) [H00055937-M01] - Detection limit for recombinant GST tagged APOM is approximately 0.3ng/ml as a capture antibody.

• Reactivity: Hu, Mu
• Applications: WB, DB, ELISA, KD
• Clone: 1F10
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

Apolipoprotein M/ApoM Antibody (1F10) - Azide and BSA Free Summary

• Description: Novus Biologicals Mouse Apolipoprotein M/ApoM Antibody (1F10) - Azide and BSA Free (H00055937-M01) is a monoclonal antibody validated for use in WB and ELISA. Anti-Apolipoprotein M/ApoM Antibody: Cited in 3 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: APOM (AAH20683, 23 a.a. ~ 188 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. CPEHSQLTTLGVDGKEFPEVHLGQWYFIAGAAPTKEELATFDPVDNIVFNMAAGSAPMQLHLRATIRMKDGLCVPRKWIYHLTEGSTDLRTEGRPDMKTELFSSSCPGGIMLNETGQGYQRFLLYNRSPHPPEKCVEEFKSLTSCLDSKAFLLTPRNQEACELSNN
• Specificity: APOM - apolipoprotein M
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: APOM
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • Dot Blot
  • ELISA
  • Knockdown Validated
  • Sandwich ELISA
  • Western Blot 1:500
• Application Notes: Antibody reactive against recombinant protein for western blot. It has also been used for RNAi validation and ELISA.

Reactivity Notes

Human. Other species not tested.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Apolipoprotein M/ApoM Antibody (1F10) - Azide and BSA Free

• Apolipoprotein M
• APOM
• apo-M
• G3A
• HSPC336
• MGC22400
• NG20
• NG20-like protein
• Protein G3a

Background

The protein encoded by this gene is an apolipoprotein and member of the lipocalin protein family. It is found associated with high density lipoproteins and to a lesser extent with low density lipoproteins and triglyceride-rich lipoproteins. The encoded protein is secreted through the plasma membrane but remains membrane-bound, where it is involved in lipid transport. Two transcript variants encoding two different isoforms have been found for this gene, but only one of them has been fully characterized.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Apolipoprotein M/ApoM Antibody (H00055937-M01)(3)

Publications using H00055937-M01

• Wei J, Yu Y, Luo GH et al. 17B-estradiol regulates the expression of apolipoprotein M through estrogen receptor a-specific binding motif in its promoter. Lipids Health Dis 2017-03-31 [PMID: 28359281]
• Zhang X, Mao S, Luo G et al. Effects of simvastatin on apolipoprotein M in vivo and in vitro. Lipids Health Dis. 2011-07-05 [PMID: 21729290]
• Zhang X, Zhu Z, Luo G et al. Liver X receptor agonist downregulates hepatic apoM expression in vivo in vitro. Biochem Biophys Res Commun;371(1):114-7. 2008-06-20 [PMID: 18413148]

Bioinformatics

• Gene Symbol: APOM
• Entrez:
  • 55937(Human)
• OMIM:
  • 606907(Human)
• Uniprot:
  • AAH20683(Human)