Apolipoprotein E/ApoE Antibody (E981) - BSA Free

Western Blot: Apolipoprotein E/ApoE Antibody (E981) [NBP3-18297] - Detection of human Apolipoprotein E/ApoE with NBP3-18297 in Western blot. Human plasma (0.1 ul and 0.01 ul) in SDS sample buffer were denatured at 70C for 10 minutes and run on SDS-page / Western blot. ApoE was detected using 1 ug/ml E981 (biotinylated) followed by SA-ALP and BCIP/NBT-plus substrate.

• Reactivity: Pm-NHP
• Applications: WB, ELISA
• Clone: E981
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 0.5 mg/ml

Apolipoprotein E/ApoE Antibody (E981) - BSA Free Summary

• Description: Novus Biologicals Mouse Apolipoprotein E/ApoE Antibody (E981) - BSA Free (NBP3-18297) is a monoclonal antibody validated for use in WB and ELISA. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Purified human apolipoprotein E/ApoE
• Specificity: Native and recombinant apolipoprotein E (isoforms apoE2, apoE3 and apoE4)
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: APOE
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Western Blot

Reactivity Notes

The monoclonal antibody reacts with human apoE and cross-react with apoE from non-human primates.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 0.2 um filtered PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Protein G purified

Alternate Names for Apolipoprotein E/ApoE Antibody (E981) - BSA Free

• APOE
• Apo-E
• Apolipoprotein E
• apolipoprotein E3
• late onset)
• MGC1571

Background

Apolipoprotein E (APOE) is an apolipoprotein located in chylomicrons and intermediate-density lipoproteins that is necessary for the normal catabolism of triglyceride-rich lipoprotein constituents in the liver. APOE has recently been shown to be involseveral biological processes other than lipoprotein transport, including cognition and immunoregulation.

Lipoproteins are responsible for carrying cholesterol and other fats through the bloodstream as little packages and are essential for the normal breakdown of these molecules. In particular, apolipoprotein E is a major component of specific lipoproteins called very low-density lipoproteins (VLDL). A major function of VLDLs is to remove excess cholesterol from the blood and carry it to the liver for processing. Maintaining normal levels of cholesterol is essential for the prevention of cardiovascular diseases, including heart attacks and strokes.

The three Apo E isoforms are strongly associated with cardiovascular disease and Alzheimer's disease, so ApoE antibodies have become useful tools for research in both of these areas.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: APOE