Rat Apolipoprotein A5 ELISA Kit (Colorimetric)

ELISA: Rat Apolipoprotein A5 ELISA Kit (Colorimetric) [NBP2-68250] - Samples were spiked with high concentrations of Rat Apolipoprotein A5 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

ELISA: Rat Apolipoprotein A5 ELISA Kit (Colorimetric) [NBP2-68250] - Standard Curve Reference

• Reactivity: Rt
• Applications: ELISA
• Suitable Sample Type: Serum, plasma and other biological fluids
• Standard Curve Range: 0.31 - 20 ng/mL
• Sensitivity: 0.19 ng/mL

Rat Apolipoprotein A5 ELISA Kit (Colorimetric) Summary

• Specificity: This kit recognizes Rat ApoA5 in samples. No significant cross-reactivity or interference between Rat ApoA5 and analogues was observed.
• Standard Curve Range: 0.31 - 20 ng/mL
• Sensitivity: 0.19 ng/mL
• Assay Type: Sandwich-ELISA
• Inter-Assay: CV% < 5.29%
• Intra-Assay: CV% < 5.12%
• Spike Recovery: 86-106%
• Sample Volume: 100 uL
• Kit Type: ELISA Kit (Colorimetric)
• Gene: APOA5

Applications/Dilutions

• Dilutions:
  • ELISA

Packaging, Storage & Formulations

• Storage: Storage of components varies. See protocol for specific instructions.

Alternate Names for Rat Apolipoprotein A5 ELISA Kit (Colorimetric)

• APOAV
• apo-AV
• APOA-V
• Apolipoprotein A5
• apolipoprotein A-V
• FLJ97995
• MGC126836
• RAP3MGC126838
• Regeneration-associated protein 3

Background

Apolipoprotein A5 (ApoA5) is a minor apolipoprotein, predominantly associated with HDL and and occasionally with VLDL and chylomicrons. ApoA-5 is a useful indicator of plasma triglyceride levels, a major risk factor for coronary artery disease.

Apo A5 is expressed in the plasma and the liver, where it is involved in the early phase of liver regeneration.

APOA5 mutations can lead to increaased susceptibility to familial hypertriglyceridemia (FHTR), a common inherited disorder where VLDL concentrations are elevated in the plasma. This disorder increases the risk of heart disease, obesity, and pancreatitis. APOA5 mutations can also cause hyperlipoproteinemia type 5 (HLPP5), characterized by increased amounts of chylomicrons/VLDL and decreased amounts of LDL/HDL in the plasma. This disorder often leads to a wide array of syndromes, including insulin-dependent diabetes mellitus, contraceptive steroids, alcohol abuse, and glycogen storage disease type 1A

ApoA5 antibodies are useful for studying lipoprotein processing and a variety of cardiac diseases.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

Publications for Apolipoprotein A5 ELISA Kit (NBP2-68250)(1)

Publication using NBP2-68250

• Huang PP, Zhu WQ, Xiao JM et al. Alterations in sorting and secretion of hepatic apoA5 induce hypertriglyceridemia due to short-term use of olanzapine Frontiers in pharmacology 2022-08-12 [PMID: 36034782] (ELISA, Human)

Bioinformatics

• Gene Symbol: APOA5