Apolipoprotein A-II/ApoA2 Antibody (395906)

• Reactivity: Hu
• Applications: WB
• Clone: 395906
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Unconjugated
• Concentration: LYOPH

Apolipoprotein A-II/ApoA2 Antibody (395906) Summary

• Immunogen: E. coli-derived recombinant human ApoA2
  Gln24-Gln100
  Accession # P02652
• Specificity: Detects human ApoA2 in direct ELISAs and Western blots. Does not cross‑react with recombinant human (rh) ApoA1 or rhApoE.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Rat
• Gene: APOA2
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Apolipoprotein A-II/ApoA2 Antibody (395906)

• Alp-2
• APOA2
• apoAII
• Apo-AII
• ApoA-II
• Apolipoprotein A2
• Apolipoprotein AII
• Apolipoprotein A-II
• Hdl-1

Background

Apolipoprotein A2 (ApoA2) is a major protein component of serum HDL. It is produced by the liver and is involved in cholesteryl ester formation and cholesterol transport from tissues to the liver. Polymorphisms of ApoA2 are associated with disorders of cholesterol and fatty acid metabolism. ApoA2 can form disulfide-linked 17 kDa homodimers and heterodimers with other apolipoproteins. Mature human ApoA2 shares 96% aa sequence identity with chimpanzee ApoA2 and 48% - 66% aa sequence identity with bovine, equine, mouse, and rat ApoA2.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Apolipoprotein A-II/ApoA2 Antibody (MAB4215)(1)

Publication using MAB4215

• Fukuhara T, Wada M, Nakamura S, Ono C, Shiokawa M, Yamamoto S, Motomura T, Okamoto T, Okuzaki D, Yamamoto M, Saito I, Wakita T, Koike K, Matsuura Y Amphipathic alpha-helices in apolipoproteins are crucial to the formation of infectious hepatitis C virus particles. PLoS Pathog, 2014-12-11;10(12):e1004534. 2014-12-11 [PMID: 25502789] (Western Blot, Human)

Bioinformatics

• Gene Symbol: APOA2
• Uniprot:
  • P02652()