Apolipoprotein A-I/ApoA1 Antibody (2083A) [mFluor Violet 500 SE]

• Reactivity: Hu
• Applications: WB, IHC, Flow, ICC/IF
• Clone: 2083A
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: mFluor Violet 500 SE

Apolipoprotein A-I/ApoA1 Antibody (2083A) [mFluor Violet 500 SE] Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: E. coli-derived recombinant human ApoA1
  Asp25-Gln267
  Accession # P02647
• Specificity: Detects human ApoA1 in direct ELISAs and Western blots.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry
  • Immunohistochemistry
  • Intracellular Staining by Flow Cytometry
  • Western Blot
• Application Notes: Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: 50mM Sodium Borate
• Preservative: 0.05% Sodium Azide

Notes

mFluor(TM) is a trademark of AAT Bioquest, Inc. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for Apolipoprotein A-I/ApoA1 Antibody (2083A) [mFluor Violet 500 SE]

• Alp-1
• APOA1
• Apo-AI
• apoA-I
• Apolipoprotein A1
• Apolipoprotein AI
• Apolipoprotein A-I
• Brp-14
• Ltw-1
• Lvtw-1
• MGC117399
• Sep-1
• Sep-2

Background

Apolipoprotein A1 (apo A1) is the major polypeptide of the human plasma high density lipoprotein (HDL). The structure and function of the apo A1 gene are of interest because of the inverse correlation shown between HDL levels and coronary heart disease (1). The 267 amino acid precursor initially undergoes intracellular co-translational proteolytic cleavage into proapoA-I. ProapoA-I is secreted from the cell and was isolated from thoracic duct lymph in the apoA-I1 isoform position. Results indicate that apo A1 is present in human plasma, and undergoes post-translational proteolytic cleavage to mature plasma apoA-I (2). Cleavage of this protein with cyanogen bromide yields four fragments designated in the order of elution from Bio-Gel P-30 as CNBr I, II, III, and IV (3).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.