APLP-1 Antibody - BSA Free

Immunohistochemistry-Paraffin: APLP-1 Antibody [NBP1-89036] - Staining in human cerebral cortex and liver tissues using anti-APLP1 antibody. Corresponding APLP1 RNA-seq data are presented for the same tissues.

Western Blot: APLP-1 Antibody [NBP1-89036] - Analysis in control (vector only transfected HEK293T lysate) and APLP1 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).

Immunohistochemistry-Paraffin: APLP-1 Antibody [NBP1-89036] - Staining of human liver shows no positivity in hepatocytes as expected.

Immunohistochemistry-Paraffin: APLP-1 Antibody [NBP1-89036] - Staining of human cerebral cortex shows moderate cytoplasmic positivity in neurons.

Immunohistochemistry-Paraffin: APLP-1 Antibody [NBP1-89036] - Staining of human endometrium shows no positivity in glandular cells as expected.

• Reactivity: Hu
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

APLP-1 Antibody - BSA Free Summary

• Immunogen: This antibody was developed against Recombinant Protein corresponding to amino acids: GTAVGDPSTRSWPPGSRVEGAEDEEEEESFPQPVDDYFVEPPQAEEEEETVPPPSSHTLAVVGKVTPTPRPTDGVDIYFGMPGEISEHEGFLRAKMDLEERRMRQINEVMREWAMADNQSK
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: APLP1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:1000 - 1:2500
  • Immunohistochemistry-Paraffin 1:1000 - 1:2500
  • Western Blot 0.04-0.4 ug/ml
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended.
• Control Peptide:

  APLP-1 Protein (NBP1-89036PEP)

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (83%), Rat (85%)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for APLP-1 Antibody - BSA Free

• amyloid beta (A4) precursor-like protein 1
• amyloid precursor-like protein 1
• amyloid-like protein 1
• APLP1
• APLP-1
• APLPAPLP-1

Background

APLP1, or Amyloid-like protein 1, contains two similar isoforms weighing approximately 72 kDa each, and is involved in postsynaptic function and signal transduction activation. Disease research is currently being conducted on APLP1 and its relation to chronic fatigue syndrome, lissencephaly, neuronitis, infertility, neurodegeneration, pharyngitis, cerebritis, and neuroblastoma. The protein is not linked to specific pathways, but it does interact with ZNF24, TK1, TSC22D1, EEF1A1, and GDF9.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: APLP1