APJ/Apelin receptor Antibody

Product Discontinued

APJ/Apelin receptor Antibody Summary

• Immunogen: Synthetic peptide, corresponding to amino acids 150-200 of Human APLNR.
• Clonality: Polyclonal
• Host: Rabbit
• Gene: APLNR
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:50-1:200
  • Immunohistochemistry 1:50-1:200
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Western Blot 1:500-1:1000
• Theoretical MW: 43 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Human.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2)
• Preservative: 0.09% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for APJ/Apelin receptor Antibody

• AGTRL1
• AGTRL1HG11
• angiotensin II receptor-like 1
• Angiotensin receptor-like 1
• apelin receptor
• APJ (apelin) receptor
• APJ receptor
• APJ
• APJFLJ96609
• APJR
• APLNR
• FLJ90771
• G protein-coupled receptor APJ
• G-protein coupled receptor APJ
• G-protein coupled receptor HG11
• HG11 orphan receptor
• HG11
• MGC45246

Background

There are at least two distinct receptor subtypes of angiotensin II: angiotensin II receptor, type 1 (AGTR1) and angiotensin II receptor, type 2 (AGTR2). Most of the effects of angiotensin II are mediated by the AGTR1 receptor. This gene is related to the AGTR1 gene by sequence similarity. It was cloned based on a conserved transmembrane domain found in members of the G protein-coupled receptor (GPCR) gene family.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

FAQs for APJ/Apelin receptor Antibody (NBP1-00713). (Showing 1 - 2 of 2 FAQ).

1. I am interested to know if NBP1-00713 recognizes an extracellular loop on the Apelin Receptor protein. Can you tell me which amino acids or an approximate number of amino acids flanking the Cysteine 181 that the antibody make recognize?
   • NBP1-00713 was raised against a synthetic peptide corresponding to a sequence which includes cysteine 181 of the human protein. According to sequence information provided on uniprot.org, the sequence containing this residue is well within an extracellular loop of the protein.
2. Can you tell me if the entire peptide that was used to generate the antibody is present in this extracellular loop?
   • Yes, the entire peptide is exclusively present in the extracellular loop. I hope this helps!

Bioinformatics

• Gene Symbol: APLNR
• Entrez:
  • 187(Human)
• Uniprot:
  • P35414()