Apc5 Antibody - BSA Free

Western Blot: Apc5 Antibody [NB100-55319] - Detection of human APC5 by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-APC5 antibody NB100-55319 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Western Blot: Apc5 Antibody [NB100-55319] - Detection of mouse APC5 by western blot. Samples: Whole cell lysate (50 ug) from TCMK-1 and NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-APC5 antibody NB100-55319 used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Immunoprecipitation: Apc5 Antibody [NB100-55319] - Detection of human APC5 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-APC5 antibody NB100-55319 (lot NB100-55319-2) used for IP at 3 ug per reaction. APC5 was also immunoprecipitated by a previous lot of this antibody (lot NB100-55319-1) and another rabbit anti-APC5 antibody. Detection: Chemiluminescence with an exposure time of 3 minutes.

• Reactivity: Hu, Mu
• Applications: WB, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

Apc5 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit Apc5 Antibody - BSA Free (NB100-55319) is a polyclonal antibody validated for use in WB and IP. Anti-Apc5 Antibody: Cited in 2 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: The immunogen recognized by this antibody maps to a region between residue 705 and the C-terminus (residue 755) of human anaphase promoting complex subunit 5 using the numbering given in entry NP_057321.2 (GeneID 51433).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: ANAPC5
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation 2-5 ug/mg lysate
  • Western Blot 1:500-1:2500
• Theoretical MW: 85 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Tris-Citrate/Phosphate (pH 7.0 - 8.0)
• Preservative: 0.09% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Apc5 Antibody - BSA Free

• anaphase promoting complex subunit 5
• anaphase-promoting complex subunit 5
• APC5Cyclosome subunit 5

Background

APC5 is a component of the anaphase-promoting complex (APC). The APC complex functions as an ubiquitin ligase that controls cell cycle progression and promotes the transition from metaphase to anaphase. The APC complex is composed of at least 11 APC components. APC5 may serve a scaffolding role that connects the enzymatic core APC subunits with the regulatory APC subunits.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Apc5 Antibody (NB100-55319)(2)

Publications using NB100-55319

• Fehr AR, Gualberto NC, Savaryn JP et al. Proteasome-dependent disruption of the E3 ubiquitin ligase anaphase-promoting complex by HCMV protein pUL21a. PLoS Pathog 2012-01-01 [PMID: 22792066]
• Tran K, Kamil JP, Coen DM et al. Inactivation and disassembly of the anaphase-promoting complex during human cytomegalovirus infection is associated with degradation of the APC5 and APC4 subunits and does not require UL97-mediated phosphorylation of Cdh1. J Virol 2010-10-01 [PMID: 20686030]

Bioinformatics

• Gene Symbol: ANAPC5
• Entrez:
  • 51433(Human)
  • 59008(Mouse)
• Uniprot:
  • NP_057321.2(Human)